小鼠心肌层注射方法的改进
收稿日期: 2017-10-30
网络出版日期: 2017-12-30
基金资助
国家自然科学基金青年基金资助项目(81700351); 常州市卫生计生委青年人才科技资助项目(QN201707)
Improvement of myocardial layer injection in mice
Received date: 2017-10-30
Online published: 2017-12-30
王惠, 赵蓉, 周学中 . 小鼠心肌层注射方法的改进[J]. 上海大学学报(自然科学版), 2017 , 23(6) : 835 . DOI: 10.12066/j.issn.1007-2861.1969
An improved myocardial layer injection acts as an effectual mode of administration was established. Used improved syringe, Evans Blue and green fluorescent protein(GFP) labeled lentivirus were injected in to mouse myocardial layer. Evans Blue and lentivirus were found to be stored in mouse myocardial layer, and GFP could stably express in the whole heart for a long time. In conclusion, an improved myocardial layer injection may be used to cardiovascular disease (CVD) research and gene therapy research because of the good uniformity and high success rate.
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