收稿日期: 2017-04-03
网络出版日期: 2017-06-30
基金资助
国家自然科学基金资助项目(81670362, 81470515); 上海市科委医学引导(重点)基金资助项目(134119a3000)
Improvement and preliminary evaluation of neonatal rat cardiac regeneration model
Received date: 2017-04-03
Online published: 2017-06-30
朱浩1, 丁胜光1, 黄海涛1, 许嘉鸿2, 仲崇俊1 . 新生大鼠心脏再生模型的改良及评价模式[J]. 上海大学学报(自然科学版), 2017 , 23(3) : 387 -394 . DOI: 10.12066/j.issn.1007-2861.1938
An improved neonatal rat cardiac regeneration model was established and a preliminary evaluation was conducted. Neonatal rats of 12 h and exercised apical resection surgery with low-temperature anesthesia were used. Postoperative survival rates were taken on 1, 3, 7 and 21 d, and the cross-sectional area were measured to evaluate the advantage of the improved technique. In the control group, postoperative survival rate at 1 d was 61%, while the improved group was 77%. In addition, survival rates of the improved group on 3, 7 and 21 d were higher than that in the control group. Variation of resected apex crosssectional area was smaller in the improved group. In conclusion, the improved neonatal rat cardiac regeneration model is more stable and more uniform with a higher operation
success rate. This model may be used to investigate genes and the signaling pathways mediating mammalian heart regeneration.
Key words： apical resection; cell proliferation; heart regeneration
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