收稿日期: 2017-12-21
网络出版日期: 2019-09-04
基金资助
国家自然科学基金资助项目(21477073);国家自然科学基金资助项目(41511140096)
Distributions of allergenic platanus pollen protein in the ambient particulates in the spring of Shanghai
Received date: 2017-12-21
Online published: 2019-09-04
选择上海大气颗粒物中悬铃木致敏花粉蛋白Pla a3为研究对象, 利用生物工程手段表达Pla a3蛋白, 并利用该致敏蛋白免疫大鼠, 制备出单一致敏原的抗体. 通过酶联免疫吸附测定法(enzyme linked immunosorbent assay, ELISA), 检测分析上海大气颗粒物中Pla a3的分布特征. 结果表明: 颗粒物中总蛋白质量浓度在$<$1.1 $\mu $m的粒径段内最高, 为2.5$\sim $3.0 $\mu $g/m$^{3}$; Pla a3蛋白主要分布在$>$7.0 $\mu $m的粒径段内, 其平均质量浓度为7.5 pg/m$^{3}$, 但在$<$1.1 $\mu $m粒径段内的质量浓度最低. 在上述分析结果基础上, 将进一步探讨颗粒物中主要有害组分与致敏蛋白分布之间的关系.
关键词： 大气颗粒物; 致敏蛋白Pla a3; 酶联免疫吸附测定法; 粒径分布
赵慧, 彭加仙, 洪强, 张红莉, 王伟倩, 王青躍, 谭正莹, 周树敏, 张卫, 吕森林 . 上海春季大气颗粒物中致敏悬铃木花粉蛋白的分布特征[J]. 上海大学学报(自然科学版), 2019 , 25(4) : 544 -549 . DOI: 10.12066/j.issn.1007-2861.2007
The allergenic pollen protein Pla a3 in Shanghai atmospheric particulate matter was selected as the research aim. Pla a3 protein from platanus pollen was expressed by biological engineering. Then rats were immunized and special antibody were prepared from the rat serum. The distribution of Pla a3 protein in Shanghai atmospheric particulate matter was maeasured and analyzed by making use of the enzyme linked immunosorbent assay (ELISA). Research results showed that total protein content in particulate matter was the highest in the particles with size diameter less than 1.1 $\mu$m. Its mass concentration is 2.5$\sim$3.0 $\mu$g/m$^3$. The Pla a3 protein in particulate matters was found mainly in particles with size diameter larger than 7.0 $\mu$m, with average mass level was 7.5 pg/m$^3$. Mass level of Pla a3 protein with size diameter less than 1.1 $\mu$m was the least. Based on the research data collected, the connection between allergenic protein and toxic composition in the ambient particles will be further explored.
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