大豆异黄酮的测定方法及其评价

上海大学学报(自然科学版) ›› 2007, Vol. 13 ›› Issue (6): 741-746 .

大豆异黄酮的测定方法及其评价

顾建明，潘春云

上海大学生命科学学院，上海 200444

收稿日期:2006-09-19 修回日期:1900-01-01 出版日期:2007-12-20 发布日期:2007-12-20
通讯作者: 顾建明

Analytical Approach of Soybean Isoflavones and Its Evaluation

GU Jian-ming,PAN Chun-yun

School of Life Sciences, Shanghai University, Shanghai 200444, China

Received:2006-09-19 Revised:1900-01-01 Online:2007-12-20 Published:2007-12-20
Contact: GU Jian-ming

摘要/Abstract

摘要：

大豆异黄酮的测定可采用单波长法、三波长法和液相色谱法.单波长法（ y=7.431 7

x-0.055 6，R2=0.999 8）和三波长法（y=32.432x+0.048 8，R2=0.998 1）用于总异黄酮测定,操作简便.高效液相色谱法（染料木甙，y=1 828.6x-96.467，R2=0.996 4；大豆甙，y=1 795.4x-420.67，R2=0.995 3）用于单个异黄酮成分的测定，操作较复杂.精密度和准确度依次为：液相色谱法>三波长法>单波长法.

关键词: 测定, 大豆异黄酮, 高效液相色谱法, 紫外光谱法

Abstract:

Soybean isoflavones were determined with a single-wave-length method, a three-wave-length method and HPLC. Singlewavelength method (y=7.431 7x-0.055 6,R2=0.999 8) and three-wave-length method (y=32.432x+0.048 8, R2=0.998 1) were used conveniently for total isoflavones. HPLC (genistin, y=1 828.6x-96.467, R2=0.996 4; daidzin, y=1 795.4x-420.67, R2=0.9953) was used for single isoflavone, which was complicated. Accuracy and precision of the three methods in a descending order is HPLC, three-wave-length method, and single-wave-length method.

顾建明;潘春云. 大豆异黄酮的测定方法及其评价[J]. 上海大学学报(自然科学版), 2007, 13(6): 741-746 .

GU Jian-ming;PAN Chun-yun. Analytical Approach of Soybean Isoflavones and Its Evaluation[J]. Journal of Shanghai University（Natural Science Edition）, 2007, 13(6): 741-746 .