BDE-47的两种代谢产物对HepG2细胞中谷胱甘肽含量和P450酶活性的影响

上海大学学报(自然科学版) ›› 2010, Vol. 16 ›› Issue (6): 587-591.

BDE-47的两种代谢产物对HepG2细胞中谷胱甘肽含量和P450酶活性的影响

（上海大学环境与化学工程学院，上海 200444）

出版日期:2010-12-21 发布日期:2010-12-21
通讯作者: 安静(1976～)，女，副研究员，博士,研究方向为环境毒理学. Email:peace74839@shu.edu.cn E-mail:peace74839@shu.edu.cn
作者简介:安静(1976～)，女，副研究员，博士,研究方向为环境毒理学. Email:peace74839@shu.edu.cn
基金资助:
国家重点基础研究发展计划（973计划）资助项目（2008CB41820）；上海市重点学科建设资助项目（S30109）

Effects of 5-hydroxylated/5-methoxylated-2,2′,4,4′tetrabromodiphenyl
Ether on Glutathione Content and Activity of Cytochrome P450 in HepG2 Cells

(School of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, China)

Online:2010-12-21 Published:2010-12-21

摘要/Abstract

摘要：

从细胞毒性、氧化应激以及代谢酶活性等方面，研究2,2′,4,4′四溴联苯醚（2,2′,4,4′tetrabromodiphenyl ether，BDE-47）的两种代谢物5羟基四溴联苯醚（5-OH-BDE-47）和5-甲氧基-四溴联苯醚（5-MeO-BDE-47）对人体肝癌细胞HepG2的细胞毒性效应.采用噻唑蓝（methyl thiazolyl tetrazolium，MTT）法，检测5-OH-BDE-47和5- MeO-BDE-47染毒后HepG2细胞的生长情况，以及急性染毒后细胞内谷胱甘肽（GSH）含量及细胞内P450酶活性的变化.研究结果表明，5-OH-BDE-47和 5-MeO-BDE-47均能抑制HepG2细胞生长，降低GSH活性，诱导EROD和PROD活性的增强.

关键词: 多溴联苯醚；2,2′,4,4′四溴联苯醚；肝癌细胞；谷胱甘肽；细胞色素

Abstract:

HepG2 cells have been used to study the possible cytotoxicity, the level of glutathione （GSH） and the activity of P450 enzymes after exposure to 5-OH-BDE-47 and 5-MeO-BDE-47. HepG2 cells proliferation was detected after 24 h, 48 h and 72 h exposure with methyl thiazolyl tetrazolium （MTT） test, GSH were measured after cells were treated with 5-OH/5-MeO-BDE47 as above mentioned doses for 24 h, P450 enzymes were tested after 48 h. After 72 h exposure to 5-OH-BDE-47 and 5-MeO-BDE-47, proliferation of HepG2 cells was inhibited. The content of GSH can be reduced by 5, 10, 20 μmol/L 5-OH-BDE-47 and only 20 μmol/L 5-MeO-BDE-47. Furthermore, PROD activity was induced by 5-OH-BDE-47 and 5-MeO-BDE-47 under all concentrations, and EROD activity was induced only under higher concentration (20 μmol/L) of 5-OH-BDE-47 and 5-MeO-BDE-47. Neither 5-OH-BDE47 nor 5-MeO-BDE-47 was capable of inducing MROD activity. These results show that both 5-OH-BDE-47 and 5-MeO-BDE-47 can inhibit HepG2 cells proliferation, reduce the level of GSH, and increase EROD and PROD activity.

Key words: polybrominated diphenyl ethers (PBDEs); BDE-47; HepG2; glutathione (GSH); cytochrome P450

尹灵灵王流林钟玉芳安静吴明红盛国英傅家谟. BDE-47的两种代谢产物对HepG2细胞中谷胱甘肽含量和P450酶活性的影响[J]. 上海大学学报(自然科学版), 2010, 16(6): 587-591.

YIN Ling-Ling, WANG Liu-Lin, ZHONG Yu-Fang, AN Jing, WU Meng-Hong, SHENG Guo-Ying, FU Jia-Mo. Effects of 5-hydroxylated/5-methoxylated-2,2′,4,4′tetrabromodiphenyl
Ether on Glutathione Content and Activity of Cytochrome P450 in HepG2 Cells[J]. Journal of Shanghai University（Natural Science Edition）, 2010, 16(6): 587-591.