6-羟基-多溴联苯醚47对A549细胞抗氧化系统的影响

上海大学学报(自然科学版) ›› 2010, Vol. 16 ›› Issue (3): 302-305.

6-羟基-多溴联苯醚47对A549细胞抗氧化系统的影响

赵金玉,钟玉芳，王艺陪，王杨君，安静，盛国英，傅家谟

（上海大学环境与化学工程学院环境污染与健康研究所，上海 200444）

出版日期:2010-06-28 发布日期:2010-06-28

Effects of 6-hydroxylated Polybrominated Diphenyl Ethers-47 on Anti-Oxidative Systems of A549 Cells

ZHAO Jin-yu,ZHONG Yu-fang,WANG Yi-pei,WANG Yang-jun,

(Institute of Environmental Pollution and Health, School of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, China)

Online:2010-06-28 Published:2010-06-28

摘要/Abstract

摘要：

多溴联苯醚(polybrominated diphenyl ethers, PBDEs)是一类具有内分泌干扰和神经毒性的持久性有机污染物，但对其代谢产物的毒性研究还较少.从细胞毒性和氧化应激的角度，探讨2,2′,4,4′四溴联苯醚（2,2′,4,4′tetrabromodiphenyl ether，BDE47）的羟基代谢物6羟基多溴联苯醚47（6hydroxylated2,2′,4,4′tetrapolybrominated diphenyl ethers， 6-OH-BDE47）对人肺癌细胞系A549的作用.研究结果发现，6-OH-BDE47对A549细胞具有明显的细胞毒性，随着染毒剂量增加和时间延长，A549细胞存活率逐渐下降.急性染毒实验证实，6-OH-BDE47对A549细胞的氧化应激系统有严重的影响.当细胞培养体系中6OH-BDE47浓度≥0.5 μmol/L时，24 h急性暴露能诱导A549细胞的总超氧化物歧化酶（total superoxide dismutase ，T-SOD）活力升高1.03倍以上；而6-OH-BDE47浓度≥2.0 μmol/L时，还原性谷胱甘肽（reduced the glutathione，GSH）含量减少71.2％.由此可见，6-OH-BDE47有一定的细胞毒性，能刺激A549细胞发生氧化应激，诱导抗氧化酶升高和内源性还原剂耗竭.

关键词: 多溴联苯醚；持久性有机污染物；氧化应激；多溴联苯醚47

Abstract:

Polybrominated diphenyl ethers (PBDEs), a class of persistent organic pollutants, were proved to be endocrine disruptors and neurotoxic agents. However, there are few reports about the toxic effects of their hydroxylated metabolites. Our objective was to investigate the cytotoxicity of 6-hydroxylated2,2′,4,4′tetrapolybrominated diphenyl ethers (6-OH-BDE47) and its effects on anti-oxidative systems in A549 cells after acute exposure. The results show that 6-OH-BDE47 has obvious cytotoxicity, and the A549 cell survival gradually decrease after exposure in a dose and time-dependent manner. Acute exposure experiment confirmed that the oxidative stress system of A549 cells was seriously impaired by 6-OH-BDE47. When the final concentration of 6-OH-BDE47 in culture medium was higher than 0.5 μmol/L, the total superoxide dismutase (T-SOD) content in treatment group cells increased by 1.03 times compared to the control group cells; and ≥ 2.0 μmol/L of 6-OH-BDE47 reduced the glutathione content by 71.2%. These results suggest that 6-OH-BDE47 has certain cytotoxicity on A549 cells and can impair oxidative stress responses as evidenced by increased SOD activity and decreased GSH.

Key words: polybrominated diphenyl ethers; persistent organic pollutants; oxidative stress; BDE47

赵金玉,钟玉芳，王艺陪，王杨君，安静，盛国英，傅家谟. 6-羟基-多溴联苯醚47对A549细胞抗氧化系统的影响[J]. 上海大学学报(自然科学版), 2010, 16(3): 302-305.

ZHAO Jin-yu,ZHONG Yu-fang,WANG Yi-pei,WANG Yang-jun,. Effects of 6-hydroxylated Polybrominated Diphenyl Ethers-47 on Anti-Oxidative Systems of A549 Cells[J]. Journal of Shanghai University（Natural Science Edition）, 2010, 16(3): 302-305.