三七毛根皂苷对动脉粥样硬化大鼠血脂及血管细胞黏附分子表达影响

上海大学学报(自然科学版) ›› 2009, Vol. 15 ›› Issue (3): 320-325.

三七毛根皂苷对动脉粥样硬化大鼠血脂及血管细胞黏附分子表达影响

胡俊沈中晨沈芸翁新楚

上海大学生命科学学院，上海 200444

出版日期:2009-06-30 发布日期:2009-06-30
通讯作者: 翁新楚（1962~），男，教授，博士生导师，博士，研究方向为天然产物资源开发、油脂抗氧化等.E-mail:wxch@staff.shu.edu.cn

Effects of PNS on Blood Lipids and mRNA Expression of Vascular Cell Adhesion Molecule-1 in Atherosclerotic Rats

HU Dun, CHEN Zhong-Chen, CHEN Yun, WENG Xin-Chu

School of Life Sciences, Shanghai University, Shanghai 200444, China

Online:2009-06-30 Published:2009-06-30

摘要/Abstract

摘要：

观察三七毛根皂苷（PNS）对实验性动脉粥样硬化（AS）大鼠血脂及血管细胞黏附分子-1（VCAM-1） mRNA表达的影响.采用高脂饲料配合腹腔维生素D3注射法建立AS大鼠模型，将Wistar大鼠随机分成正常对照组、模型组、PNS低剂量组、PNS高剂量组、阳性对照组.治疗4周后检测血脂水平、血清丙二醛（MDA）含量及超氧化物歧化酶（SOD）活力，分离主动脉，苏木素伊红（HematoxylinEosin）染色及RTPCR技术检测主动脉VCAMl mRNA表达.模型组大鼠均出现血脂异常及主动脉病理形态改变.PNS低、高剂量组能降低TC,TG,LDL-c及MDA含量，升高HDL-c及SOD活力（P<0.01或P<0.05），下调VCAM-l基因mRNA的表达（P<0.01）.表明PNS具有良好的抗AS作用，其下调相关基因VCAM-1 mRNA的表达可能是其产生治疗作用的分子机理之一.

关键词: 三七毛根粗总皂苷；动脉粥样硬化模型；血管细胞黏附因子-1

Abstract:

The effect of Panax notoginseng (PNS) on blood lipids and expression of aorta vascular cell adhesion moleculel of experimental atherosclerotic rats was studied. Atherosclerotic rats were replicated by administration of excessive vitamin D3 followed by feeding a highcholesterol diet. Experimental rats were divided into normal control group, model group, PNS low dosage group, PNS high dosage group, and simvastatin group. After treatment for 4 weeks, cholesterol (TC), triglyceride (TG), low density lipoprotein (LDLc), high density lipoprotein (HDLc), malondialdehyde (MDA) level and activity of superoxide dismutase (SOD) were detected. Aorta alteration of tissue morphology was observed by HematoxylinEosin staining. Half-quantitative reverse transcription polymerase chain reaction (RT-PCR) was used to detect VCAM-1 gene expression in artery vessel. High and lowdosages of PNS were significantly reduce serum TC, TG, LDLc and MDA levels, elevate HDLc and SOD activity in atherosclerotic rats (P<0.01 or P<0.05). The histopathological observation of aortic arch showed alleviation of atherosclerotic damage by PNS. VCAM1 mRNA expression was significantly inhibited by PNS (P<0.01). PNS can regulate down the gene expression of VCAM-1 in artery vessels of experimental atherosclerotic rats, which may account for the antiatherosclerosis effects of PNS.

Key words: Panax notoginseng(PNS); atherosclerosis; vascular cell adhesion molecule-1

中图分类号:

R966

胡俊沈中晨沈芸翁新楚. 三七毛根皂苷对动脉粥样硬化大鼠血脂及血管细胞黏附分子表达影响[J]. 上海大学学报(自然科学版), 2009, 15(3): 320-325.

HU Dun, CHEN Zhong-Chen, CHEN Yun, WENG Xin-Chu. Effects of PNS on Blood Lipids and mRNA Expression of Vascular Cell Adhesion Molecule-1 in Atherosclerotic Rats[J]. Journal of Shanghai University（Natural Science Edition）, 2009, 15(3): 320-325.