镉离子胁迫下4种辣椒超氧化物歧化酶-同工酶酶谱及酶活性分析

上海大学学报(自然科学版) ›› 2009, Vol. 15 ›› Issue (2): 216-220.

• 生命科学 • 上一篇

镉离子胁迫下4种辣椒超氧化物歧化酶-同工酶酶谱及酶活性分析

邓志瑞罗琳

(上海大学生命科学学院,上海 200444)

收稿日期:2007-11-06 出版日期:2009-04-22 发布日期:2009-04-21
通讯作者: 邓志瑞(1964～),男，副教授，博士，研究方向为植物生理与分子生物学. E-mail:dengzhirui@staff.shu.edu.cn
作者简介:邓志瑞(1964～),男，副教授，博士，研究方向为植物生理与分子生物学. Email：dengzhirui@staff.shu.edu.cn
基金资助:
上海市高等学校科学技术发展基金资助项目（207105）

Catalytic Activity and Isozyme Patterns of Superoxide Dismutase in Four Species of Capsicums under Cd²⁺ Stress

DENG Zhirui,LUO Lin

(School of Life Sciences, Shanghai University, Shanghai 200444, China)

Received:2007-11-06 Online:2009-04-22 Published:2009-04-21

摘要/Abstract

摘要：

以4种辣椒为材料,分析在不同胁迫浓度和胁迫时间镉离子胁迫下,4种辣椒超氧化物歧化酶(SOD)活性及同工酶酶谱的变化.结果表明,镉离子能显著影响酶活性的变化,不同镉离子浓度胁迫下不同材料酶活性变化不同, SOD酶活性最高为对照组的1.4倍(1号品种,100 mg/L胁迫3 d),酶活性最低的为对照组的60%(2号品种,150 mg/L胁迫6 d);镉离子还能影响同工酶酶谱,不同胁迫时间和浓度对同工酶的影响不同,对1号材料的影响最为显著,能诱导出2条SOD同工酶新谱带.

关键词: 辣椒；镉；超氧化物歧化酶(SOD)；同工酶；酶谱；酶活性

Abstract:

Catalytic activity and isozyme patterns of superoxide dismutase (SOD) were studied in four species of capsicums at different times under series concentration of cadmium (Cd). The results show that Cd²⁺ can affect catalytic activity of SOD remarkably and dissimilarly according to its concentration. The highest catalytic activity is 1.4 times higher compared to the control (No.1 species, 100 mg/L for 3 d) while the lowest is only 60% of that of control
(No.2 species, 150 mg/L for 6 d). Besides, Cd²⁺ can also affect isozyme patterns of SOD, especially in No.1 species. Two new isozyme bands are induced.

Key words: capsicums; cadmium; superoxide dismutase(SOD); isoenzyme; isozyme patterns; catalytic activity

中图分类号:

Q 554+.9

邓志瑞罗琳. 镉离子胁迫下4种辣椒超氧化物歧化酶-同工酶酶谱及酶活性分析[J]. 上海大学学报(自然科学版), 2009, 15(2): 216-220.

DENG Zhi-Rui, LUO Lin. Catalytic Activity and Isozyme Patterns of Superoxide Dismutase in Four Species of Capsicums under Cd²⁺ Stress[J]. Journal of Shanghai University（Natural Science Edition）, 2009, 15(2): 216-220.

镉离子胁迫下4种辣椒超氧化物歧化酶-同工酶酶谱及酶活性分析

Catalytic Activity and Isozyme Patterns of Superoxide Dismutase in Four Species of Capsicums under Cd²⁺ Stress

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镉离子胁迫下4种辣椒超氧化物歧化酶-同工酶酶谱及酶活性分析

Catalytic Activity and Isozyme Patterns of Superoxide Dismutase in Four Species of Capsicums under Cd2+ Stress

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Catalytic Activity and Isozyme Patterns of Superoxide Dismutase in Four Species of Capsicums under Cd²⁺ Stress