TMV重组外壳蛋白Sc1754CP的原核表达和多克隆抗体制备

上海大学学报(自然科学版)

TMV重组外壳蛋白Sc1754CP的原核表达和多克隆抗体制备

李蓉，宋任涛，许政暟，李平

上海大学生命科学学院，上海 200444

收稿日期:2007-04-19 修回日期:1900-01-01 出版日期:2008-06-30 发布日期:2008-06-30
通讯作者: 李-平

Expression of Sc1754CP and Preparation of Anti-Sc1754CP Antibody

LI Rong,SONG Ren-tao,XU Zheng-kai,LI Ping

School of Life Sciences, Shanghai University, Shanghai 200444, China

Received:2007-04-19 Revised:1900-01-01 Online:2008-06-30 Published:2008-06-30
Contact: LI Ping

摘要/Abstract

摘要： 烟草花叶病毒（tobacco mosaic virus，TMV）重组外壳蛋白（coat protein，CP）Sc1754CP是一种能够激发敏感烟草产生过敏性反应（hypersensitive reaction, HR）的蛋白, 但是这种蛋白无法通过植物病毒的大量扩增获得.为了获得足够量的上述蛋白，并对这种蛋白在植物中激发的HR反应进行深入研究，作者在大肠杆菌中大量表达Sc1754CP蛋白，并且获得了Sc1754CP高度专一性的多克隆抗体.上述研究为进一步深入研究Sc1754CP及其激发的新的过敏性反应机制奠定了基础.

关键词: Sc1754CP, 多克隆抗体
, 原核表达

Abstract: The recombinant coat protein of TMVSc1754 can induce hypersensitive reaction in susceptible tobacco. However this protein hardly is accumulated in the inoculated plants. In order to clarify the role of Sc1754CP in this hypersensitive reaction, we overexpressed Sc1754CP in E.coli. Polyclonal AntiSc1754CP rabbit polyclonal antibody has been successfully prepared. The Sc1754CP protein and the antibody against Sc1754CP can help us further study this unknown incompatible reaction between pathogen and host.

Key words: polyclonal antibody, prokaryotic expression, Sc1754CP

中图分类号:

Q 7854

李蓉;宋任涛;许政暟;李平. TMV重组外壳蛋白Sc1754CP的原核表达和多克隆抗体制备[J]. 上海大学学报(自然科学版).

LI Rong;SONG Ren-tao;XU Zheng-kai;LI Ping. Expression of Sc1754CP and Preparation of Anti-Sc1754CP Antibody
[J]. Journal of Shanghai University（Natural Science Edition）.