CREB在家蚕中枢神经系统中的免疫组织化学定位

上海大学学报(自然科学版)

CREB在家蚕中枢神经系统中的免疫组织化学定位

张扬，沈琦，沈欣，宋红生

上海大学生命科学学院，上海 200444

收稿日期:2006-12-15 修回日期:1900-01-01 出版日期:2008-04-30 发布日期:2008-04-30
通讯作者: 宋红生

Immunohistochemical Localization of CREB in the Central Nervous

ZHANG Yang, SHEN Qi, SHEN Xin, SONG Hong-sheng

School of Life Sciences, Shanghai University, Shanghai 200444, China

Received:2006-12-15 Revised:1900-01-01 Online:2008-04-30 Published:2008-04-30
Contact: SONG Hong-sheng

摘要/Abstract

摘要： 用免疫组织化学的方法，研究了CREB蛋白在家蚕中枢神经系统中的分布.结果表明，家蚕CREB蛋白在5龄幼虫期和蛹期的中枢神经系统中均有表达，主要分布在脑和神经节的胞体密集区.从5龄幼虫期到蛹期，随着发育的进行，CREB阳性细胞数逐渐减少.比较二化性品种的滞育系与非滞育系，CREB蛋白在5龄幼虫脑中的表达分布存在明显的差异，滞育系的阳性反应细胞比非滞育系多.这些结果暗示，CREB蛋白可能参与家蚕生长发育、变态的调控以及环境条件调控家蚕滞育的过程.

关键词: CREB, 家蚕, 免疫组织化学, 滞育

Abstract: Using CREB antibody, immunostaining was performed on the central nervous system (CNS) of silkworm, Bombyx mori. CREB immunoreactivity was distributed in some neurons of CNS in the larval and the pupal stages. The numbers of CREB immunopositive neurons decrease from the 5th instar to the pupation. CREB immunoreactivity was different between diapause producer and nondaipause producer of bivoltine strain. Many immunopositive neurons were found in the 5th instar larval brain of diapause producer, but not in the brain of nondiapause producer. These results indicate that CREB may be related to regulation of the development and the metamorphosis of silkworm, and may play some role in the diapause process induced by environments.

Key words: Bombyx mori, diapause, immunohistochemistry, CREB

中图分类号:

Q786

张扬;沈琦;沈欣;宋红生. CREB在家蚕中枢神经系统中的免疫组织化学定位[J]. 上海大学学报(自然科学版).

ZHANG Yang;SHEN Qi;SHEN Xin;SONG Hong-sheng. Immunohistochemical Localization of CREB in the Central Nervous

[J]. Journal of Shanghai University（Natural Science Edition）.