应用毛细管电泳表征噬菌体与丹酚酸B的相互作用

上海大学学报(自然科学版)

应用毛细管电泳表征噬菌体与丹酚酸B的相互作用

刘书朋1,3，孙烨1，郭薇2，徐宇虹2

1.上海交通大学生命科学技术学院，上海 200240；
2.上海交通大学药学院，上海 200240；
3.上海大学通信与信息工程学院，上海 200072)

收稿日期:2006-11-24 修回日期:1900-01-01 出版日期:2008-02-28 发布日期:2008-02-28
通讯作者: 徐宇虹

Characterization of Interaction between Salvianolic Acid B and Phages Using Capillary Electrophoresis

LIU Shu-peng1,3, SUN Ye1, GUO Wei2, XU Yu-hong2

1. College of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China; 
2. School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China; 
3. School of Communication and Information Engineering, Shanghai University, Shanghai 200072, China

Received:2006-11-24 Revised:1900-01-01 Online:2008-02-28 Published:2008-02-28
Contact: XU Yu-hong

摘要/Abstract

摘要： 基于Hummel-Dreyer毛细管电泳方法研究经噬菌体展示库生物淘洗亲和筛选出的噬菌体和丹酚酸B之间的结合作用，结合系数由PRISM软件中Scatchard方程曲线拟合计算得出.实验结果表明，筛选出的该噬菌体克隆的表面蛋白和丹酚酸B具有特异性的结合作用，也说明利用毛细管电泳测定噬菌体和丹酚酸B的结合系数是可行的.

关键词: 丹酚酸B, 毛细管电泳, 噬菌体

Abstract: Binding activity between Salvianolic acid B and selected phages from affinity biopanning in phage display library were studied with capillary lectrophoresis based on the Hummel-Dreyer methods. The binding constant was calculated with Scatchard analysis using PRISM software. The results indicate that the surface proteins expressed by the phage clone interact specifically with Salvianolic acid B, and it is feasible to determine the binding constant between the selected phage and Salvianolic acid B using CE.

Key words: capillary electrophoresis, phage, Salvianolic acid B

中图分类号:

刘书朋;孙烨;郭薇;徐宇虹. 应用毛细管电泳表征噬菌体与丹酚酸B的相互作用[J]. 上海大学学报(自然科学版).

LIU Shu-peng;SUN Ye;GUO Wei;XU Yu-hong. Characterization of Interaction between Salvianolic Acid B and Phages Using Capillary Electrophoresis [J]. Journal of Shanghai University（Natural Science Edition）.

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