English

上海大学学报(自然科学版)

• 生命科学 • 上一篇    

抗稻瘟病水稻BAC文库的构建与鉴定

陈琪1,2，邓一文3，黄彬彬1,2，孙晓明1,2，张文文1,2，王刚1,2，许政暟1,2,3，宋任涛1,2   

  1. 1.上海大学 生命科学学院，上海 200444；
    2.上海市能源作物培育及利用重点实验室，上海 200444；
    3.中科院 上海植物生理生态研究所，上海 200032
  • 收稿日期:2006-08-30 修回日期:1900-01-01 出版日期:2007-06-30 发布日期:2007-06-30

Construction and Characterization of a Bacterial Artificial Chromosome Library from Blast Resistance Rice

CHEN Qi1,2,　DENG Yi-wen3,　HUANG Bin-bin1,2,　SUN Xiao-ming1,2,　ZHANG Wen-wen1,2,　WANG Gang1,2,　XUN Zheng-kai1,2,3,　SONG Ren-tao1,2   

  1. 1. School of Life Sciences, Shanghai University, Shanghai 200444, China;
    2. Shanghai Key Laboratory of Bioenergy Corp, Shanghai University, Shanghai 200444, China; 
    3. Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai 200032, China
  • Received:2006-08-30 Revised:1900-01-01 Online:2007-06-30 Published:2007-06-30

被引次数

16

摘要： 水稻是一种重要的粮食作物，同时也是一种重要的单子叶植物模式.稻瘟病是水稻生长中的一种严重病害，因此分离和克隆新的稻瘟病抗病基因具有重要的应用价值.以一个抗稻瘟病农家种水稻为材料，以plndigoBAC5为载体，构建了细菌人工染色体（BAC）文库.该文库共有90 000个转化子，插入频率99％，插入片段平均长度为105 kb，由此推测这个文库覆盖水稻基因组约20倍.利用其中的45 000个转化子建立了4维PCR筛选体系，并且通过4维PCR筛选体系，筛选获得了7个含水稻分蘖基因（MOC1）的阳性克隆.因此该文库是一个高质量、高覆盖率的水稻BAC文库，能有效用于目的基因的分离.

关键词: 4维PCR筛选体系, 稻瘟病, 水稻, 细菌人工染色体文库

Abstract: Rice blast is a serious disease in production. Therefore the isolation of novel blast resistance gene is important. We constructed a bacterial artificial chromosome (BAC) library from a rice verity with blast resistance with vector plndigoBAC5. The BAC library consisted of about 90 000 clones, 99% of the which had insertion with an average insertion size of about 105 kb. The BAC library was estimated to have 20 times genome coverage. Half of the library consisting of about 45 000 clones was used to develop a 4-dimensional PCR screening system, through which 7 positive MOCI clones were obtained. The result indicats that this rice BAC library has high quality and high coverage, and is sufficient for target gene isolation.

Key words: 4-dimentional screening system, BAC, rice blast, rice

中图分类号: 

© 2019 上海大学学报(自然科学版)编辑部
办公地址：上海市宝山区南陈路333号上海大学东区3#楼221室电话：021-66135508　 传真：021-66132736　 E-mail： xuebao@mail.shu.edu.cn
通信地址：上海市上大路99号上海大学126信箱 邮编：200444
本系统由北京玛格泰克科技发展有限公司设计开发 技术支持：support@magtech.com.cn