上海大学学报(自然科学版) ›› 2015, Vol. 21 ›› Issue (2): 220-228.doi: 10.3969/j.issn.1007-2861.2014.03.001

• 生命科学 • 上一篇    下一篇

隔山消总黄酮的提取工艺优化及其抗氧化特性

杨申明1,2, 王应顺1,2, 王振吉1,2   

  1. (1. 楚雄师范学院化学与生命科学学院, 云南楚雄675000;2. 云南省高校应用生物学重点实验室, 云南楚雄675000)
  • 收稿日期:2013-10-24 出版日期:2015-04-29 发布日期:2015-04-29
  • 通讯作者: 王振吉(1983—), 男, 副教授, 研究方向为生理生化. E-mail:wangzj@cxtc.edu.cn
  • 基金资助:

    国家自然科学基金资助项目(31300370); 云南省应用基础计划资助项目(2011FZ186); 云南省青年应用基础计划资助项目(2012FD049); 云南省重点建设学科资助项目(05YJJSXK03); 云南省高校科技创新团队支持计划资助项目(IRTSTYN); 楚雄师范学院重点建设学科基金资助项目(05YJJSXK03

Optimizing extraction technology of total flavonoids in Cynanchum wilfordii and its antioxidant effects

YANG Shen-ming1,2, WANG Ying-shun1,2, WANG Zhen-ji1,2   

  1. (1. Department of Chemistry and Life Science, Chuxiong Normal University,Chuxiong 675000, Yunnan, China;2. Yunnan Province Universities Key Laboratory of Applied Biology, Chuxiong 675000, Yunnan, China)
  • Received:2013-10-24 Online:2015-04-29 Published:2015-04-29

摘要: 采用乙醇回流法提取隔山消(Cynanchum wilfordii)的总黄酮, 通过正交实验优化了总黄酮的提取工艺, 并就总黄酮对活性氧自由基的清除作用进行了初步研究. 结果表明: 总黄酮的最佳提取工艺参数为乙醇体积分数60%, 提取温度75℃, 提取时间60 min, 料液比1∶30(g/mL); 在此条件下总黄酮的平均提取率为10.79%, 平均加样回收率为98.78%; 总黄酮对羟基自由基(·OH)和超氧阴离子自由基(O2 ·)的清除率可分别达到29.2%和36.7%, 说明总黄酮对·OH和O2 ·有较好的清除能力.

关键词: 超氧阴离子自由基, 隔山消, 羟基自由基, 正交实验, 总黄酮, 超氧阴离子自由基, 隔山消, 羟基自由基, 正交实验, 总黄酮

Abstract: Total flavonoids in Cynanchum wilfordii were extracted using an ethanol refluxing method, and the extraction procedure was optimized with orthogonal experiments. The antioxidant effects of the obtained total flavonoids on active oxygen radicals were studied. The results show the following optimum extraction parameters. The volume fraction of ethanol as extractant is 60%, the extraction temperature is 75℃, the extraction time is 60 min, and the ratio of solid to liquid is 1∶30 (g/mL). Under these conditions, the average extraction rate of total flavonoids reaches 10.79%, the average recovery rate of the added sample is 98.78%, and the scavenging effects of total flavonoids on ·OH and O2 · are 29.2% and 36.7%, respectively, implying that the material has good ability to scavenge ·OH and O2 ·.

Key words: Cynanchum wilfordii, hydroxyl radical, orthogonal experiment, superoxide anion free radical, total flavonoid, Cynanchum wilfordii, hydroxyl radical, orthogonal experiment, superoxide anion free radical, total flavonoid

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