四川乐山大佛地衣与苔藓群落中的微生物多样性

doi:10.12066/j.issn.1007-2861.2295

上海大学学报(自然科学版) ›› 2023, Vol. 29 ›› Issue (1): 166-174.doi: 10.12066/j.issn.1007-2861.2295

四川乐山大佛地衣与苔藓群落中的微生物多样性

陈学萍¹^,², 白法妍¹, 余娟¹, 陆永生¹, 宋绍雷²^,³, 董海燕², 彭学艺⁴, 黄继忠²()

1.上海大学环境与化学工程学院, 上海 200444
2.上海大学文化遗产保护基础科学研究院, 上海 200444
3.上海大学纳米科学与技术研究中心, 上海 200444
4.乐山大佛乌尤文物保护管理局, 四川乐山 614003

出版日期:2023-02-28 发布日期:2023-03-28
通讯作者: 黄继忠 E-mail:hjizhong@163.com
作者简介:黄继忠(1965|), 男, 研究员, 博士生导师, 博士, 研究方向为岩土文物保护. E-mail: hjizhong@163.com
基金资助:
国家重点研发计划资助项目(2019YFC1520500)

Microbial diversity in lichen and moss habitats on Leshan Giant Buddha in Sichuan, China

CHEN Xueping¹^,², BAI Fayan¹, YU Juan¹, LU Yongsheng¹, SONG Shaolei²^,³, DONG Haiyan², PENG Xueyi⁴, HUANG Jizhong²()

1. School of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, China
2. Institute for the Conservation of Cultural Heritage, Shanghai University, Shanghai 200444, China
3. Nano-Science and Technology Research Center, Shanghai University, Shanghai 200444, China
4. Wuyou Cultural Heritage Protection Bureau of Leshan Giant Buddha, Leshan 614003, Sichuan, China

Online:2023-02-28 Published:2023-03-28
Contact: HUANG Jizhong E-mail:hjizhong@163.com

摘要/Abstract

摘要：

为了探究生物侵蚀微生物的种类, 通过高通量测序手段对乐山大佛地衣(lichen, LI)和苔藓样本进行了微生物(细菌、古菌和真菌)多样性分析. 结果发现: 地衣、活苔藓(living moss, LM)和苔藓残体(dead moss residue, DM)样本中的细菌和真菌组成在组内样本之间能进行聚类, 组间差异也能良好区分; 在细菌群落中, 地衣与活苔藓样本中的细菌组成较为相似, 两个样本的优势细菌均为蓝细菌(Cyanobacteria)和醋酸菌(Acetobacteraceae); 在真菌群落中, 地衣样本的优势真菌是煤炱目(Capnodiales), 但其在活苔藓样本中含量较少, 活苔藓样本的优势真菌是子囊菌门(Ascomycota), 且其在地衣和苔藓残体样本中也有一定含量(7.47%$\sim $52.6%); 古菌群落的组内差异较大, 其中与氮转化相关的泉古菌(soil Crenarchaeotic group, SCG)总体丰度占比较高, 并发现了较多未归类古菌(unclassified-k-norank).

关键词: 石质文物, 生物侵蚀, 活苔藓, 地衣, 苔藓残体, 微生物, 生物处理

Abstract:

Microbial diversity in lichen (LI) and moss habitats on Leshan Giant Buddha was analysed using high-throughput sequencing to determine the major microorganisms responsible for the biodeterioration of this monument. Bacterial and fungal communities were clustered in the LI, live moss (LM), and dead moss residue (DM) groups. The communities could be distinguished among the three groups. The bacterial communities in LI and LM groups were similar, with the families Cyanobacteria and Acetobacteraceae predominating. The order Capnodiales was the dominant fungal order in the LI group, but it was less abundant in the LM group. Ascomycota was the dominant fungal phylum in the LM group. The relative abundance ranged from 7.47% to 52.6% in the LI and DM groups. The archaeal community markedly differed among LI, LM, and DM groups. The soil Crenarchaeotic group (SCG) related to nitrogen transformation was the most abundant. In addition, large numbers of unclassified-k-norank species were observed across all groups.

Key words: rock heritage, biodeterioration, living moss(LM), lichen(LI), dead moss residue(DM), microorganism, biological treatment

中图分类号:

X172

陈学萍, 白法妍, 余娟, 陆永生, 宋绍雷, 董海燕, 彭学艺, 黄继忠. 四川乐山大佛地衣与苔藓群落中的微生物多样性[J]. 上海大学学报(自然科学版), 2023, 29(1): 166-174.

CHEN Xueping, BAI Fayan, YU Juan, LU Yongsheng, SONG Shaolei, DONG Haiyan, PENG Xueyi, HUANG Jizhong. Microbial diversity in lichen and moss habitats on Leshan Giant Buddha in Sichuan, China[J]. Journal of Shanghai University（Natural Science Edition）, 2023, 29(1): 166-174.

图/表 4

参考文献 17

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四川乐山大佛地衣与苔藓群落中的微生物多样性

Microbial diversity in lichen and moss habitats on Leshan Giant Buddha in Sichuan, China

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