在线液相色谱-气相色谱/质谱联用快速分析烟叶中的西柏三烯二醇

doi:10.12066/j.issn.1007-2861.2078

上海大学学报(自然科学版) ›› 2020, Vol. 26 ›› Issue (2): 227-233.doi: 10.12066/j.issn.1007-2861.2078

在线液相色谱-气相色谱/质谱联用快速分析烟叶中的西柏三烯二醇

黄志¹, 毕艳玖¹, 沙云菲¹(), 谢雯燕¹, 吴达¹, 刘百战¹, 刘太昂²

^1. 上海烟草集团技术中心, 上海 200082
^2. 上海大学材料科学与工程学院, 上海 200444

收稿日期:2018-08-15 出版日期:2020-04-30 发布日期:2020-04-29
通讯作者: 沙云菲 E-mail:shayf@sh.tobacco.com.cn
基金资助:
国家重点研究发展计划专项资助项目(2016YFB0700504);中国烟草总公司科技重大专项资助项目(中烟办[2016]259号)

Analysis of cembratriene-diols in tobacco by LC-GC/MS

HUANG Zhi¹, BI Yanjiu¹, SHA Yunfei¹(), XIE Wenyan¹, WU Da¹, LIU Baizhan¹, LIU Taiang²

^1. Technical Center, Shanghai Tobacco Group Co., Ltd., Shanghai 200082, China
^2. School of Materials Science and Engineering, Shanghai University, Shanghai 200444, China

Received:2018-08-15 Online:2020-04-30 Published:2020-04-29
Contact: SHA Yunfei E-mail:shayf@sh.tobacco.com.cn

摘要/Abstract

摘要：

西柏三烯二醇是重要的中性香味物质——茄酮及其衍生物的前体物,对烟叶的香气风格和品质具有重要影响. 建立了以超声波辅助溶剂萃取技术,结合在线液相色谱-气相色谱/质谱(liquid chromatography-gaschromatography/mass spectrometry,LC-GC/MS)联用法对烟草中的西柏三烯二醇进行快速分析的方法.该方法的前处理操作快速简便, 检测灵敏度高, 重复性好,在对不同地区 K326 青烟叶中西柏三烯二醇含量的比较分析中也取得了良好的结果,适用于烟草中西柏三烯二醇含量的大规模分析.

关键词: 烟叶, 西柏三烯二醇, 硅烷化衍生, 液相色谱-气相色谱/质谱

Abstract:

Cembratriene-diols are the precursors of important neutral aroma components and their derivatives, which affect significantly the aroma style and tobacco quality. In this study, a method of in-series combination of ultrasound-assisted extraction and online liquid chromatography-gas chromatography/mass spectrometry (LC-GC/MS) was constructed for the analysis of cembratriene-diols in tobacco. Cembratriene-diols were first extracted by ultrasound-assisted extraction and then on-line clean-up and analysis by online LC-GC/MS system. The pretreatment was highly efficient and easy-handling. Good recovery and reproducibility had also been achieved. This method was subsequently applied to the analysis of cembratriene-diols extracted from different regions of tobacco leaves. The satisfactory performance indicated that this strategy was suitable for the high-throughput analysis of cembratriene-diols in tobacco.

Key words: tobacco leaf, cembratriene-diols, silylation derivatization, liquid chromatography-gas chromatography/mass spectrometry (LC-GC/MS)

中图分类号:

O657.63

黄志, 毕艳玖, 沙云菲, 谢雯燕, 吴达, 刘百战, 刘太昂. 在线液相色谱-气相色谱/质谱联用快速分析烟叶中的西柏三烯二醇[J]. 上海大学学报(自然科学版), 2020, 26(2): 227-233.

HUANG Zhi, BI Yanjiu, SHA Yunfei, XIE Wenyan, WU Da, LIU Baizhan, LIU Taiang. Analysis of cembratriene-diols in tobacco by LC-GC/MS[J]. Journal of Shanghai University（Natural Science Edition）, 2020, 26(2): 227-233.

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在线液相色谱-气相色谱/质谱联用快速分析烟叶中的西柏三烯二醇

Analysis of cembratriene-diols in tobacco by LC-GC/MS

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