微/纳多级结构TiO2涂层的制备及生物学性能

doi:10.12066/j.issn.1007-2861.1921

上海大学学报(自然科学版) ›› 2018, Vol. 24 ›› Issue (1): 44-55.doi: 10.12066/j.issn.1007-2861.1921

微/纳多级结构TiO₂涂层的制备及生物学性能

顾静萍¹, 谢有桃², 黄利平², 贺延昌², 赵君², 郑学斌², 李红¹(), 孙晋良¹

^1. 上海大学材料科学与工程学院, 上海 200444
^2. 中国科学院上海硅酸盐研究所特种无机涂层重点实验室,上海 200050

收稿日期:2017-03-29 出版日期:2018-02-28 发布日期:2018-03-05
通讯作者: 李红 E-mail:lihong2007@shu.edu.cn
基金资助:
国家自然科学青年基金资助项目(51502328)

Fabrication and biological properties of micro/nano hybrid structured TiO₂coatings

GU Jingping¹, XIE Youtao², HUANG Liping², HE Yanchang², ZHAO Jun², ZHENG Xuebin², LI Hong¹(), SUN Jinliang¹

^1. School of Materials Science and Engineering,Shanghai University, Shanghai 200444, China
^2. Key Laboratory of Inorganic Coating Materials, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, China

Received:2017-03-29 Online:2018-02-28 Published:2018-03-05
Contact: LI Hong E-mail:lihong2007@shu.edu.cn

摘要/Abstract

摘要：

表面形貌是影响骨植入体稳定性的重要因素,仿生微/纳多级结构表面能加快早期细胞响应,加速植入体与骨组织间的整合, 并改善其长期稳定性.以锐钛矿相二氧化钛悬浮液为喂料, 采用液相感应等离子喷涂(induction suspension plasma spraying,ISPS)技术制备微/纳多级结构TiO$_{2}$涂层,并对其细胞相容性进行了研究. 扫描透射电子显微镜(scanning transmission electron microscopy, SEM)观察结果表明,通过对进料速率的调控, 可制备典型微/纳多级结构的TiO$_{2}$涂层,气孔率随进料速率的增大而增大. X射线衍射(X-ray diffraction,XRD)结果表明, 涂层以金红石相为主, 并含少量锐钛矿相,金红石相含量随进料速率的增大而降低,这可能与等离子体喷涂的高温过程有关,锐钛矿相二氧化钛在此过程中发生相变, 产生了更为稳定的金红石相.通过体外细胞相容性检测发现,ISPS制备的微/纳多级结构TiO$_{2}$涂层具有较高的蛋白吸附水平,成骨细胞在其表面能较好铺展, 碱性磷酸酶(alkaline phosphatase,ALP)活性、矿化等基因表达均高于大气等离子喷涂涂层和光滑钛表面.

关键词: 纳米二氧化钛, 液相感应等离子喷涂, 微/纳多级结构, 细胞相容性

Abstract:

Micro/nano hybrid structured surface can improve their capability of encouraging early cell responses and rapid bone in-growth. The micro/nano hybrid structured TiO$_{2}$ coatings were prepared by induction suspension plasma spraying (ISPS) using anatase TiO$_{2}$ suspension as a feedstock. Their osteogenesis related cell behaviors were studied. Scanning transmission electron microscopy (SEM) results show that the coatings prepared by adjusting feed rate have typical micro/nano hybrid structures. Porosity of the coating increases with the increase of feed rate. The X-ray diffraction (XRD) results show that the coating is mainly composed of rutile with a small amount of anatase, and the content of rutile decreased with the increase of feed rate. This may be related to the high temperature process of plasma spraying, in which the anatase phase is transformed into a more stable rutile phase. The results of in vitro cell compatibility tests show that the micro/nano structured TiO$_{2}$ coatings prepared with ISPS has higher protein adsorption, better osteoblasts spreading and higher expression of alkaline phosphatase (ALP) activity and mineralization than atmospheric plasma sprayed coatings and polished titanium surfaces.

Key words: nano titania, induction suspension plasma spraying, micro/nano hybrid structure, cell compatibility

中图分类号:

TB321

顾静萍, 谢有桃, 黄利平, 贺延昌, 赵君, 郑学斌, 李红, 孙晋良. 微/纳多级结构TiO₂涂层的制备及生物学性能[J]. 上海大学学报(自然科学版), 2018, 24(1): 44-55.

GU Jingping, XIE Youtao, HUANG Liping, HE Yanchang, ZHAO Jun, ZHENG Xuebin, LI Hong, SUN Jinliang. Fabrication and biological properties of micro/nano hybrid structured TiO₂coatings[J]. Journal of Shanghai University（Natural Science Edition）, 2018, 24(1): 44-55.

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微/纳多级结构TiO₂涂层的制备及生物学性能

Fabrication and biological properties of micro/nano hybrid structured TiO₂coatings

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