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Journal of Shanghai University >

2018 , Vol. 24 >Issue 1: 74 - 82

DOI: https://doi.org/10.12066/j.issn.1007-2861.1846

Research Articles

Reaction of 1-chloro-3-buten-2-one with N-acetylcysteine

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  • School of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, China

Received date: 2016-10-25

  Online published: 2018-03-05

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Abstract

1-Chloro-3-buten-2-one (CBO) is an in vitro metabolite of 1,3-butadiene, a carcinogenic pollutant.CBO is cytotoxic and genotoxic, and can readily form DNA adducts and glutathione (GSH) conjugates. CBO-GSH conjugates are expected to be biotransformed into CBO-N-acetylcysteine (NAC) ones in the kidney, which are excreted in the urine. Thus, CBO-NAC conjugates can be used as biomarkers. However, the reaction of CBO with NAC has not been investigated yet. In the present study, this reaction under in vitro physiological conditions (pH=7.4, 37 ℃) was investigated and two products, which were structurally characterized by 1D/2D nuclear magnetic resonance (NMR) spectroscopy as 1-chloro-4-(N-acetyl-S-cysteinyl)-2-butanone and 1,4-bis(N-acetyl-S-cysteinyl)-2-butanone, were observed. The latter is a novel compound. Identification of the two CBO-NAC conjugates will help future investigation on the in vivo formation of CBO.

Key words： 1-chloro-3-buten-2-one; N-acetylcysteine; 1; 3-butadiene; metabolite; biomarker

Cite this article

LIU Boliang, ZHANG Xinyu . Reaction of 1-chloro-3-buten-2-one with N-acetylcysteine[J]. Journal of Shanghai University, 2018 , 24(1) : 74 -82 . DOI: 10.12066/j.issn.1007-2861.1846

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