The content of glucose detection has important significance in many fields and sensor technology has been applied in our daily life. Therefore glucose sensor becomes a hot topic in the study of biological sensors. In this article, four transition metal oxides of nanomaterial modified carbon paste electrodes were applied to detect glucose. The electrolyte concentration and the influence of NiO content in the modified materials were also studied. High electric potential was used in processing the electrode to prove electrochemical performance of non-enzyme glucose sensor. This study lays a foundation for applications of non-enzyme electrochemical glucose sensors.