Lactobacillus casei LC2W was irradiated by low frequency pulsed magnetic field (LFPMF) with maximum strength of 5 T. The effect of LFPMF on the cell growth and cell activity were investigated. Some correlation index of bacteria cell membrane irradiated by LFPME were also detected, including the resistance
to ultrasonic, cell membrane permeability to carboxyfluorescein diacetate succinimidyl ester (CFDA-SE) and the composition of the fatty acids. The results suggest that LFPMF can inhibit growth of the cells and reduce the activity of cells. The survival rate of the cells irradiated by LFPMF increased by 37.9% after ultrasonic processing by 30 min, the permeability of the cell membrane reduced by 20.3%, the unsaturated fatty acids of the cell membrane was increased from 1.53 to 2.07. The results indicate that LFPMF has significant effect on the composition and structure of LC2W’s cell wall and cell membrane.
ZHANG Yi-kai1, LIAO Xian-yan1, WU Zheng-jun2, HOU Jian-ping2, WENG Xin-chu1
. Effect of Low Frequency Pulsed Magnetic Field on Lactobacillus casei LC2W[J]. Journal of Shanghai University, 2013
, 19(6)
: 641
-646
.
DOI: 10.3969/j.issn.1007-2861.2013.06.017
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