The effects of different cadmium ion concentrations on callus induction, growth and plantlet regeneration of creeping bentgrass were studied. When cadmium ion concentration in medium was below 2.50 mg/L, the induction frequency was higher than that of control. The induction frequency reached up to 83.3% when cadmium ion concentration in medium was 2.50 mg/L, indicating that low cadmium ion concentration may have stimulating effect on callus formation. As cadmium ion concentration increased, the induction frequency remarkably declined and callus formation was completely inhibited when cadmium ion concentration was over 50.00 mg/L. In the early days of the callus growth (0~30 d), the calli on the medium containing 12.50 mg/L cadmium ion were morphologically largest, and further prolonged growth (30~45 d) exhibited that one lower concentration (2.50 mg/L) gave stronger stimulating effect. During regeneration, regenerated seedlings grew best on the medium where 1.25 mg/L cadmium ion concerntration existed.
DENG Yi-fei, DAI Jia-ying, WU Wen-jun, ZHANG Wen-ju, CHEN Qin, DENG Zhi-rui
. Effects of Cadmium on Callus Induction and Regeneration from Mature Embryos of Creeping Bentgrasses[J]. Journal of Shanghai University, 2013
, 19(6)
: 636
-640
.
DOI: 10.3969/j.issn.1007-2861.2013.06.016
[1] 朱雅安, 陈智勇, 易自力. 几种匍匐翦股颖愈伤组织再生系统的建立[J]. 湖南林业科技, 2004, 31(2): 26-27.
[2] 詹振楠, 刘宛, 孙梨宗, 等. 镉胁迫对拟南芥幼苗基因组DNA 多态性的影响[J]. 生态学杂志, 2011, 30(6): 1234-1239.
[3] 潘秀, 刘福春, 柴民伟. 镉在互花米草中积累、转运及亚细胞的分布[J]. 生态学杂志, 2012, 31(3): 526-531.
[4] 胡彦, 陈沁, 邓志瑞, 等. 辣椒光合系统对重金属Cd2+胁迫的响应[J]. 上海大学学报: 自然科学版, 2005, 11(5): 531-534.
[5] 邓志瑞, 罗琳. 镉离子胁迫下4 种辣椒超氧化物歧化酶同工酶酶谱及活性分析[J]. 上海大学学报: 自然科学版, 2009, 15(2): 216-220.
[6] 梁俊, 赵政阳, 樊明涛. 陕西渭北苹果园土壤中汞、镉污染与分布特征研究[J]. 农业工程学报, 2008, 24(3): 209-213.
[7] 谢惠玲, 陈爱萍, 张凤英. 紫苏对不同浓度镉胁迫的生理响应[J]. 中国生态农业学报, 2011, 19(3): 672-675.
[8] 徐向华, 徐德福, 王晓蓉, 等. 玉米幼苗对镉、菲单一与复合污染的生理生化响应[J]. 环境科学, 2011, 32(5): 1471-1476.
[9] 李子芳, 刘惠芬, 熊肖霞, 等. 镉胁迫对小麦种子萌发幼苗生长及生理生化特性的影响[J]. 农业环境科学学报, 2005, 24: 17-20.
[10] de Livera J, Mike J M, Ganga M, et al. Cadmium solubility in paddy soils: effects of soil oxidation, metal sulfides and competitive ions [J]. Science of the Total Environment, 2011, 409(8): 1489-1497.
[11] 张义贤, 李晓科. 镉、铅及其复合污染对大麦幼苗部分生理指标的影响[J]. 植物研究, 2008, 28(1): 43-46.
[12] 张利强. 水稻重金属镉的吸收、转运和积累特性研究[D]. 北京: 中国农业科学院, 2012: 79.
[13] 曾泉, 徐子勤. 几种匍匐翦股颖成熟胚愈伤组织诱导及植株再生[J]. 西北植物学报, 2006, 26(10): 2033-2038.
[14] Murashige T, Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures [J]. Physiologia Plantarum, 1962, 15: 473-497.
[15] 周晓星. 柳属植物对重金属镉胁迫的生长与生理响应[D]. 北京: 中国林业科学研究院, 2012: 113.
[16] Daud M K, Sun Y, Dawood M, et al. Cadmiuminduced functional and ultrastructural alterations in roots of two transgenic cotton cultivars [J]. Journal of Hazardous Materials, 2009, 161(1): 463-473.
[17] Wu F B, Dong J, Jia G X, et al. Genotypic difference in the responses of seedling growth and Cd toxicity in rice (Oryza sativa L.) [J]. Agricultural Sciences in China, 2006, 5(1): 68-76.
[18] 刘俊. 镉胁迫下大豆生长发育的生理生态动态特征研究[D]. 长沙: 湖南农业大学, 2010:
