Life Science

Isolation, Sequence Analysis and Prokaryotic Expression of a Putative Transcriptional Factor ZmbHLH5 from Maize (Zea mays)

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  • Shanghai Key Laboratory of Bio-energy Crops, School of Life Sciences, Shanghai University, Shanghai 200444, China

Online published: 2013-12-30

Abstract

Based on expressed sequence tags (ESTs) isolated from the cDNA library of maize developing kernels, a full-length putative basic helix-loop-helix (bHLH) transcription factor, named ZmbHLH5 was cloned successfully. Sequence analysis showed that ZmbHLH5 cDNA was 1 389 bp in length with an open reading frame (ORF) encoding 211 amino acids and untranslated regions (UTR) of 329 and 433 bp at the 3′and 50-ends, respectively. The protein encoded by ZmbHLH5 had high homology with bHLH transcription factors of other plants, ranging from 68% to 87%, with a conserved 60 amino acids bHLH domain. Expression pattern demonstrated that ZmbHLH5 was expressed at high level in ear and early developing kernels, and at least three ZmbHLH5 isoforms were produced by alternative splicing during kernel development. The fused protein GST-ZmbHLH5 was successfully expressed in prokaryotic expression system with an expected molecular weight of 58 kD.

Cite this article

WANG Fang, ZHONG Ming-yu, SONG Ren-tao, XU Zheng-kai, WANG Gui-feng . Isolation, Sequence Analysis and Prokaryotic Expression of a Putative Transcriptional Factor ZmbHLH5 from Maize (Zea mays)[J]. Journal of Shanghai University, 2013 , 19(6) : 623 -630 . DOI: 10.3969/j.issn.1007-2861.2013.06.014

References

[1] Massart M E, Murre C. Helix-loop-helix proteins: regulators of transcription in eucaryotic organisms [J]. Mol Cell Biol, 2000, 20(2): 429-440.

[2] Kong Q, Pattanaik S, Feller A, et al. Regulatory switch enforced by basic helix-loop-helix and ACTdomain mediated dimerizations of the maize transcription factor R [J]. Proc Natl Acad Sci USA, 2012, 109(30): E2091-E2097.

[3] Toledo-Ortiz G, Huq E, Quail P H. The Arabidopsis basic/helix-loop-helix transcription factor family [J]. Plant Cell, 2003, 15(8): 1749-1770.

[4] 朱玉贤, 李毅, 郑晓峰. 现代分子生物学[M]. 3 版. 北京: 高等教育出版社, 2008: 305-307.

[5] Bailey P C, Martin C, Toledo-Ortiz G, et al. Update on the basic helix-loop-helix transcription factor gene family in Arabidopsis thaliana [J]. Plant Cell, 2003, 15(11): 2497-2502.

[6] Li X, Duan X, Jiang H, et al. Genome-wide analysis of basic/helix-loop-helix transcription factor family in rice and Arabidopsis [J]. Plant Physiol, 2006, 141(4): 1167-1184.

[7] Heim M A, Jakoby M, Werber M, et al. The basic helix-loop-helix transcription factor family in plants: a genome-wide study of protein structure and functional diversity [J]. Mol Biol Evol, 2003, 20(5): 735-747.

[8] Murakami M, Ashikari M, Miura K, et al. The evolutionarily conserved OsPRR quintet: rice pseudo-response regulators implicated in circadian rhythm [J]. Plant Cell Physiol, 2003, 44(11): 1229-1236.

[9] Ludwig S R, Habera L F, Dellaporta S L, et al. Lc, a member of the maize R gene family responsible for tissue-specific anthocyanin production, encodes a protein similar to transcriptional activators and contains the myc-homology region [J]. Proc Natl Acad Sci USA, 1989, 86(18): 7092-7096.

[10] Petroni K, Cominelli E, Consonni G, et al. The developmental expression of the maize regulatory gene Hopi determines germination-dependent anthocyanin accumulation [J]. Genetics, 2000, 155(1): 323-336.

[11] Yi K, Wu Z, Zhou J, et al. OsPTF1, a novel transcription factor involved in tolerance to phosphate starvation in rice [J]. Plant Physiol, 2005, 138(4): 2087-2096.

[12] Zhang F, Gonzalez A, Zhao M, et al. A network of redundant bHLH proteins functions in all TTG1-dependent pathways of Arabidopsis [J]. Development, 2003, 130(20): 4859-4869.

[13] Penfield S, Josse E M, Kannangara R, et al. Cold and light control seed germination through the bHLH transcription factor SPATULA [J]. Curr Biol, 2005, 15(22): 1998-2006.

[14] Kondou Y, Nakazawa M, Kawashima M, et al. Retarded growth of EMBRYO1, a new basic helix loop-helix protein, expresses in endosperm to control embryo growth [J]. Plant Physiol, 2008, 147(4): 1924-1935.

[15] Strable J, Scanlon M J. Maize (Zea mays): a model organism for basic and applied research in plant biology [J]. Cold Spring Harb Protoc, 2009, doi: 10.1101/pdb. emo132.

[16] Carpita N C, Mccann M C. Maize and sorghum: genetic resources for bioenergy grasses [J]. Trends Plant Sci, 2008, 13(8): 415-420.

[17] Motto M, Hartings H, Fracassetti M, et al. Grain quality-related traits in maize: gene identification and exploitation [J]. Maydica, 2011, 56(3): 291.

[18] Wang G, Wang H, Zhu J, et al. An expression analysis of 57 transcription factors derived from ESTs of developing seeds in maize (Zea mays) [J]. Plant Cell Rep, 2010, 29(6): 545-559.

[19] Wang G,Wang F, Zhang X, et al. Opaque1 encodes a myosin Ⅺmotor protein that is required for endoplasmic reticulum motility and protein body formation in maize endosperm [J]. Plant Cell, 2012, 24(8): 3447-3462.

[20] Wang G F, Zhang X W, Wang F, et al. Isolation of high quality RNA from cereal seeds containing high levels of starch [J]. Phytochem Anal, 2012, 23(2): 159-163.
[21] Debernardi J M, Rodriguez R E, Mecchia M A, et al. Functional specialization of the plant miR396 regulatory network through distinct microRNA target interactions [J]. PLoS Genet, 2012, 8(1): e1002419.

[22] Atchley W R, Fitch W M. A natural classification of the basic helix-loop-helix class of transcription factors [J]. Proc Natl Acad Sci USA, 1997, 94(10): 5172-5176.

[23] Friedrichsen D M, Nemhauser J, Muramitsu T, et al. Three redundant brassinosteroid early response genes encode putative bHLH transcription factors required for normal growth [J]. Genetics, 2002, 162(3): 1445-1456.

[24] Lorrain S, Allen T, Duek P D, et al. Phytochrome-mediated inhibition of shade avoidance involves degradation of growth-promoting bHLH transcription factors [J]. Plant J, 2008, 53(2): 312-323.

[25] Bou-Torrent J, Roig-Villanova I, Galstyan A, et al. PAR1 and PAR2 integrate shade and hormone transcriptional networks [J]. Plant Signal Behav, 2008, 3(7): 453-454.

[26] Brioudes F, Joly C, Szecsi J, et al. Jasmonate controls late development stages of petal growth in Arabidopsis thaliana [J]. Plant J, 2009, 60(6): 1070-1080.

[27] Heang D, Sassa H. Antagonistic actions of HLH/bHLH proteins are involved in grain length and weight in rice [J]. PLoS One, 2012, 7(2): e31325.

[28] Kalsotra A, Cooper T A. Functional consequences of developmentally regulated alternative splicing [J]. Nat Rev Genet, 2011, 12(10): 715-729.

[29] Baek J M, Han P, Iandolino A, et al. Characterization and comparison of intron structure and alternative splicing between Medicago truncatula, Populus trichocarpa, Arabidopsis and rice [J]. Plant Mol Biol, 2008, 67(5): 499-510.

[30] Parra-Unda R, Vaca-Paniagua F, Jimenez L, et al. Cu, Zn superoxide dismutase: cloning and analysis of the Taenia solium gene and Taenia crassiceps

cDNA [J]. Exp Parasitol, 2012, 130(1): 32-38.
[31] Miao L X, Cao Z J, Shen C, et al. Alternative splicing of breast cancer associated gene BRCA1 from breast cancer cell line [J]. J Biochem Mol Biol, 2007, 40(1): 15-21.

[32] Kitamura-Abe S, Itoh H, Washio T, et al. Characterization of the splice sites in GT-AG and GCAG introns in higher eukaryotes using full-length cDNAs [J]. J Bioinform Comput Biol, 2004, 2(2): 309-331.

[33] Procissi A, Piazza P, Tonelli C. A maize r1 gene is regulated post-transcriptionally by differential splicing of its leader [J]. Plant Mol Biol, 2002, 49(2): 239-248.

[34] James A B, Syed N H, Bordage S, et al. Alternative splicing mediates responses of the Arabidopsis circadian clock to temperature changes [J]. Plant Cell, 2012, 24(3): 961-981.

[35] Li Y, Humbert S, Howell S H. ZmbZIP60 mRNA is spliced in maize in response to ER stress [J]. BMC Res Notes, 2012, 5: 144.
 
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