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Journal of Shanghai University >

2019 , Vol. 25 >Issue 4: 550 - 557

DOI: https://doi.org/10.12066/j.issn.1007-2861.1963

Research Articles

Cytotoxicity in SH-SY5Y cells induced by $O_{3}$-oxidized black carbon particles

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  • Institute of Environmental Pollution and Health, School of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, China

Received date: 2017-07-12

  Online published: 2019-09-04

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Abstract

SH-SY5Y cells were used to investigate neurotoxicity of O$_3$-oxidized black carbon (OBC) particles. Cell viability was measured by MTT assay after being treated with OBC for 24 and 48 h. Lactate dehydrogenase (LDH) leakage was determined to evaluate cellular membrane injury after OBC treatment for 24 h. Apoptosis rate was detected by cytometry after OBC treatment for 24 h. Concentrations of Ca$^{2+}$ was then measured with fluorescent probe of Fluo 3-AM. OBC exposure caused significant dose-dependent and time-dependent viability decrease. LC$_{50}$ for 24 and 48 h were 202.2 and 133.3 $\mu $g/mL, respectively. The LDH leakage, apoptosis rate and intracellular Ca$^{2+}$ levels were also increased significantly in a dose-dependent manner after being treated with OBC in SH-SY5Y cells. These results suggest that OBC may mediate cytotoxic effects and apoptosis through Ca$^{2+}$ release.

Key words： black carbon (BC) particles; SH-SY5Y cells; cytotoxicity; apoptosis; Ca$^{2+}$

Cite this article

Yu SHANG, Tiantian WANG, Meiying WU, Lu WANG, Huixin HE, Jing AN . Cytotoxicity in SH-SY5Y cells induced by $O_{3}$-oxidized black carbon particles[J]. Journal of Shanghai University, 2019 , 25(4) : 550 -557 . DOI: 10.12066/j.issn.1007-2861.1963

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