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Journal of Shanghai University >

2019 , Vol. 25 >Issue 4: 544 - 549

DOI: https://doi.org/10.12066/j.issn.1007-2861.2007

Research Articles

Distributions of allergenic platanus pollen protein in the ambient particulates in the spring of Shanghai

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  • 1. School of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, China
    2. School of Life Sciences, Shanghai University, Shanghai 200444, China
    3. School of Science and Engineering, Saitama University, Saitama 338-8570, Japan

Received date: 2017-12-21

  Online published: 2019-09-04

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Abstract

The allergenic pollen protein Pla a3 in Shanghai atmospheric particulate matter was selected as the research aim. Pla a3 protein from platanus pollen was expressed by biological engineering. Then rats were immunized and special antibody were prepared from the rat serum. The distribution of Pla a3 protein in Shanghai atmospheric particulate matter was maeasured and analyzed by making use of the enzyme linked immunosorbent assay (ELISA). Research results showed that total protein content in particulate matter was the highest in the particles with size diameter less than 1.1 $\mu$m. Its mass concentration is 2.5$\sim$3.0 $\mu$g/m$^3$. The Pla a3 protein in particulate matters was found mainly in particles with size diameter larger than 7.0 $\mu$m, with average mass level was 7.5 pg/m$^3$. Mass level of Pla a3 protein with size diameter less than 1.1 $\mu$m was the least. Based on the research data collected, the connection between allergenic protein and toxic composition in the ambient particles will be further explored.

Key words： atmospheric particulate matter; allergenic protein Pla a3; enzyme linked immunosorbent assay (ELISA); size distribution

Cite this article

Hui ZHAO, Jiaxian PENG, Qiang HONG, Hongli ZHANG, Weiqian WANG, Qingyao WANG, Zhengying TAN, Shumin ZHOU, Wei ZHANG, Senlin LÜ . Distributions of allergenic platanus pollen protein in the ambient particulates in the spring of Shanghai[J]. Journal of Shanghai University, 2019 , 25(4) : 544 -549 . DOI: 10.12066/j.issn.1007-2861.2007

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