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Journal of Shanghai University（Natural Science Edition） ›› 2006, Vol. 12 ›› Issue (2): 211-215 .

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Purification of Cytosolic APX from Barley Leaves, Preparation of Antibodies and Effect of AMD on APX Activities

CHEN Qin1,WANG Wei1,LIU You-liang2   

  1. 1. School of Life Sciences, Shanghai University, Shanghai 200444, China; 
    2. Faculty of Life Science, Nanjing Agriculture University, Nanjing 210095, China

  • Received:2005-05-25 Revised:1900-01-01 Online:1900-01-01 Published:1900-01-01
  • Contact: CHEN Qin1

Cited

1

Abstract:

Ascorbate peroxidase (APX，EC1.11.1.11) was purified to homogeneity from barley (Hordeum vulgare L.) leaves. APX was determined as a monomer with a molecular mass of about 29 kD by SDS-polyacrylamide gel electrophoresis. Western blot analysis using polyclonal antibodies raised against barley APX revealed that there exists immune cross-reaction of APX between Jian 4 and Kepin 7, and APX contents of two barley cultivars differing in NaCl sensitivity changed in the course of NaCl stress. Changes in APX contents showed the same trends as that of APX activities. APX activities in leaves of barley seedlings were inhibited by Actinomycin D (AMD) at different concentration.

Key words: actinomycin D (AMD)

, antibody, barley, NaCl stress, Western blot,

ascorbate peroxidase (APX)