收稿日期: 2016-01-12
网络出版日期: 2016-04-30
基金资助
国家自然科学基金资助项目(21107068, 41561144007)；上海大学长江学者和创新团队发展计划资助项目(IRT13078)
Combined effects of 1-nitropyrene and benzo(a)pyrene on cytotoxicity and DNA damage in human lung epithelial A549 cells
1-硝基芘(1-nitropyrene, 1-NP)是柴油车尾气中检测浓度最高的硝基多环芳烃. 以1-NP 为主体, 人肺上皮A549 细胞为研究对象, 探讨了1-NP 和苯并[a] 芘(benzo(a)pyrene, B[a]p)的联合细胞毒性及其对DNA 的损伤. 联合染毒实验结果表明: 当B[a]p 和1-NP 同时作用于A549 细胞时, B[a]p 能明显减弱由1-NP 造成的细胞增殖抑制和细胞内活性氧簇(reactive oxygen species, ROS)水平升高, 但对DNA 的损伤与1-NP 单独染毒组相近；利用B[a]p 预染毒A549 细胞24 h, 能明显减弱由1-NP 造成的细胞增殖抑制和ROS 水平升高, 但对DNA 的损伤加剧. 以上结果说明, 1-NP 和B[a]p 联合作用可能是通过抑制ROS 的生成来降低对细胞增殖的抑制, 但所造成的对DNA 损伤的加剧可能是通过其他的作用途径.
尚羽, 周倩, 蒋玉婷 . 1-硝基芘和苯并[a] 芘对人肺上皮A549 细胞的联合细胞毒性[J]. 上海大学学报(自然科学版), 2016 , 22(2) : 181 -187 . DOI: 10.3969/j.issn.1007-2861.2016.01.008
Using human lung epithelial A549 cells, combined toxic effects of 1-nitropyrene (1-NP) and benzo(a)pyrene (B[a]p) were evaluated. The 1-NP caused a significantly concentration-dependent viability decrease, DNA damage and reactive oxygen species (ROS) generation. Compared with the groups treated with 1-NP alone, viability was significantly increased and ROS generation was significantly reduced in combined-treated groups with 1-NP and B[a]p. However, the DNA damage was significantly increased in the combinedtreated groups compared with the groups treated with 1-NP alone. These results suggested that 1-NP may mediate the cytotoxic effects through ROS generation, and pretreatment, with B[a]p may inhibit ROS generation induced by 1-NP, and thereby reducing the cell death in A549 cells. However mechanisms of DNA damage deserves further investigations.
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