采用低频脉冲磁场(low frequency pulsed magnetic field, LFPMF)对干酪乳杆菌 LC2W 进行照射, 照射的最大强度为 5 T. 观察了磁场照射对乳杆菌菌体的生长和活力的影响, 检测了磁场作用后菌体细胞膜的相关指标, 包括菌体对超声波的抵抗性、细胞膜对羧基荧光素二乙酸盐琥珀酰亚胺酯 (carboxyfluorescein diacetate succinimidyl ester, CFDA-SE) 的通透性以及脂肪酸各组分的组成. 实验结果表明: LFPMF 对菌体的生长具有抑制作用, 且经磁场照射后的菌体活力均有所降低; LFPMF 作用后的菌体经超声波处理 30 min 后其存活率增加了37.9%, 细胞膜通透性降低了 20.3%, 细胞膜的脂肪酸不饱和度由 1.53 提高至 2.07, 说明 LFPMF 对 LC2W 的细胞壁和细胞膜的组成和结构具有显著的影响.
Lactobacillus casei LC2W was irradiated by low frequency pulsed magnetic field (LFPMF) with maximum strength of 5 T. The effect of LFPMF on the cell growth and cell activity were investigated. Some correlation index of bacteria cell membrane irradiated by LFPME were also detected, including the resistance
to ultrasonic, cell membrane permeability to carboxyfluorescein diacetate succinimidyl ester (CFDA-SE) and the composition of the fatty acids. The results suggest that LFPMF can inhibit growth of the cells and reduce the activity of cells. The survival rate of the cells irradiated by LFPMF increased by 37.9% after ultrasonic processing by 30 min, the permeability of the cell membrane reduced by 20.3%, the unsaturated fatty acids of the cell membrane was increased from 1.53 to 2.07. The results indicate that LFPMF has significant effect on the composition and structure of LC2W’s cell wall and cell membrane.
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