以吴淞口复合污染湿地土壤为研究背景, 在含有芘、柴油、Pb 的模拟复合污染湿地土壤环境中, 选取对芘具有高效降解作用的芹菜根, 研究芹菜根对复合污染湿地土壤进行生态修复的作用效果及特征. 检测分析了单一与复合污染土壤中多酚氧化酶(polyphenol oxidase, PPO)、过氧化氢酶(catalase, CAT)、脲酶(urease, URE)及不同实验组中芘的降解效果随时间的变化规律. 研究表明, 在芘、Pb、柴油复合污染胁迫下, 芹菜根可以通过根系分泌酶等一些化学物质或者通过刺激其根际微生物细胞分泌各种酶类物质, 来提高其根际微生物的活性, 促进芹菜根与其根际微生物的协同作用, 增强对复合污染土壤中芘的降解效果.
关键词：
复合污染; 降解作用; 酶; 芘; 芹菜根
This paper studies combined polluted wetland soils in Wusong estuary. Celery root with efficient degradation effect for pyrene was selected in combined pollution soil with pyrene, Pb and diesel in simulated wetland environment. The bioremediation effects and characteristics of the combined polluted wetland soil with
celery root were studied by simulation. The degradation effect on single and combined pollutants in the soil with polyphenol oxidase (PPO), catalase (CAT), urease (URE) and pyrene in different experimental groups with variation at different time were analyzed. It has been shown that celery root can improve the degradation effect of pyrene in the soil under pyrene, Pb and diesel combined pollution stress by secreting enzymes and some chemical substances. Celery root may secrete a variety of enzymes by stimulating rhizosphere microbial cells as well. It would improve activity of rhizosphere microorganisms in the soil and promote microbial synergy between celery root and its rhizosphere microorganisms. The degradation effects of pyrene in combined pollution can
also be enhanced.
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