Cupriavidus metallidurans (C.metallidurans) CH34是一种重金属耐受性细菌，能在以苯酚、甲苯酚、苯甲酸、苯胺等芳香族化合物为唯一碳源和能源的培养基中生长，其基因组中含有2个苯酚降解基因簇.以载体pIndigoBAC 5构建C.metallidurans CH34的细菌人工染色体（bacterial artificial chromosome，BAC）文库，获得约3万个克隆，平均插入片段大小为30 kb,插入频率为98%，推测该文库覆盖CH34基因组约1 240倍.用PCR筛选文库中的3 000个单克隆，共获得9个阳性克隆，其中5个克隆含有长基因簇，4个含有短基因簇，并从中得到含有全长苯酚降解基因簇的克隆.利用以苯酚为唯一碳源的无机盐培养基，研究2个基因簇在大肠杆菌中的表达情况.结果显示，两个基因簇均表现出了苯酚降解能力，短簇的降酚能力要优于长簇.

Cupriavidus metallidurans (C.metallidurans) CH34 is a kind of heavy metalresistant bacterium, whose genome own two phenoldegrading gene clusters and can grow on the medium that has phenol, cresol, benzoic acid, aniline as sole carbon and energy source. By using vector pIndigoBAC 5, a bacterial artificial chromosome (BAC) library of C.metallidurans CH34 was constructed and thirty thousand clones were obtained, from which 98% of the clones had an insertion fragment with average size of 30 kb. It was deduced that the whole BAC library size is about 1 240 times of the genome size. More than 3 000 clones were screened with PCR and nine positive clones were obtained: five with longer gene clusters and four with shorter clusters. Two clones with total length of phenoldegrading gene cluster were used to study its expression in Escherichia coli (E.coli) on the medium using phenol as the sole carbon source. The results indicate that both clones have the ability to degrade phenol, and the one carrying a shorter cluster shows stronger ability than that of the one with longer cluster.