以苏丹草(Sorghum sudanense) 种子为材料，以蛋白质提取率和电泳图谱效果为标准，分析不同提取剂、种子粉末质量（g）与提取剂体积（mL）的比例、还原剂二硫苏糖醇（dithiothreito,DTT）的浓度对种子醇溶蛋白提取效果的影响，同时研究上样量、不同胶浓度对SDS-PAGE检测蛋白的影响.结果表明，苏丹草的最佳提取剂为含有0.2%DTT的70%正丙醇，种子粉末质量与提取剂体积的最佳比例为1:8.在上样量适当的情况下，12%分离胶电泳可以得到清晰的电泳图谱.以苏丹草种子为材料所建立的醇溶蛋白提取和电泳条件，也可应用于其他几种常见的禾本科种子醇溶蛋白的分析，并可得到较好的分辨率.

This paper studies the effects of different extraction solution, ratio of sampleweight to extractionsolution and concentrations of reduction reagent dithiothreito (DTT) on extraction efficiency and quality of electrophoresis of prolamin from seeds of Sorghum sudanense. Contribution of the gel concentration and loading dosage to the results of SDSPAGE  were analyzed by evaluating the electrophoresis. The results show that 70% propylalcohol supplemented with 0.2% DTT is the best extraction solution and the ratio of sampleweight to extractionsolution 1∶8 (g/mL) can give optimal results. SDSPAGE of 12% produces the best clearance for electrophoresis under a loading appropriate sample. Established condition based on prolamin from seeds of Sorghum sudanense can be applied to isolation and electrophoresis for prolamins from several other gramineae seeds with good results.