聚乙二醇修饰多壁碳纳米管对质粒DNA的影响

劳文艳, 商迎辉, 焦正, 劳凤学

doi:doi:10.3969/j.issn.1007-2861.2011.03.018

上海大学学报(自然科学版) >

2011 , Vol. 17 >Issue 3: 302 - 306

DOI: https://doi.org/doi:10.3969/j.issn.1007-2861.2011.03.018

环境与化学工程

聚乙二醇修饰多壁碳纳米管对质粒DNA的影响

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1.北京联合大学北苑校区，北京 100012; 2.上海大学环境与化学工程学院，上海 200444

收稿日期: 2011-02-08

网络出版日期: 2011-06-24

基金资助

中国博士后科学基金资助项目（20080440213）

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Effect of Polyethylene Glycol Multiwalled Carbon Nanotubes to Plasmid DNA

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1. Beiyuan Campus, Beijing Union University, Beijing 100012, China;
2. School of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, China

Received date: 2011-02-08

Online published: 2011-06-24

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摘要

利用体外评价方法(plasmid DNA assay)和原子力显微镜(atomic force microscopy,AFM)直接观测相结合，研究原始多壁碳纳米管(multiwalled carbon nanotubes, MWNTs)、纯化后的MWNTs以及聚乙二醇（polyethylene glycol, PEG）修饰的MWNTs与DNA的相互作用.结果表明:原始MWNTs对质粒的损伤较为严重；纯化后的MWNTs对质粒的损伤有所减弱；而经PEG修饰的MWNTs对质粒的损伤非常小，表现出良好的生物相容性.

关键词： DNA; 多壁碳纳米管; 生物安全性

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劳文艳, 商迎辉, 焦正, 劳凤学 . 聚乙二醇修饰多壁碳纳米管对质粒DNA的影响[J]. 上海大学学报(自然科学版), 2011 , 17(3) : 302 -306 . DOI: doi:10.3969/j.issn.1007-2861.2011.03.018

Abstract

The biocompatibility of pristine multiwalled carbon nanotubes (MWNTs), purified MWNTs and polyethylene glycol MWNTs (PEGMWNTs) was studied using the method of plasmid DNA assay and atomic force microscopy (AFM). The results show that pristine MWNTs can cause serious damage to plasmid DNA. After purification, MWNTs may cause less damage to plasmid DNA, while at the same concentration level, PEGMWNTs cause the smallest damage to plasmid DNA.

Key words： biosafety; DNA; multi-walled carbon nanotubes (MWNTs)

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