粪大肠菌群是水质微生物污染的指示性指标. 由于常规污水工艺需要进行加氯消毒处理, 达标后排放, 故增加了成本和工艺复杂度. 以挂膜填料增强型移动生物膜反应器(enhanced mobile biofilm reactor, EMBR) 处理生物实验室废水, 评估粪大肠菌群的去除效果. 研究结果表明: 未经紫外 (ultraviolet, UV) 消毒处理, 挂膜填料对粪大肠菌群的去除效果达到 95%以上; 系统出水中粪大肠菌群和大肠杆菌噬菌体的去除率能达到 99.9% 以上, 大肠杆菌噬菌体平均滴度为 22.2±38.5 PFU/mL; 细菌总数从均值 10⁷ CFU/mL 降到 10³ CFU/mL, 出水实现一级 A 排放标准. 出水安全性测试结果显示, 斑马鱼 24 h 存活率为 100%. 可见, EMBR工艺能够实现生物实验室废水的安全性排放, 具有广泛的应用潜力.

Fecal coliform serves as an indicator of microbial contamination in water and, consequently, water quality. Chlorine disinfection is required to meet effluent standards for conventional sewage treatment; however, it incurs high costs and poses stability risks. This study purified waste effluent from a biological laboratory using an enhanced mobile biofilm reactor (EMBR) filled with biofilm carriers and investigated the removal efficiency of fecal coliforms. Results showed that the biocarrier flask, without ultraviolet (UV) treatment, achieved a 95% removal rate. Notably, removal rates exceeded 99.9% for both fecal coliforms and E.coli phages in the EMBR effluent, with an average phage titer of only 22.2±38.5 PFU/mL. The total bacterial concentration decreased from an average of 10⁷ to 10³ CFU/mL. Effluent quality met Class 1 A discharge limits. Furthermore, the biological safety test indicated a 100% survival rate of zebrafish after 24 h. These findings demonstrate that the EMBR process enables biosafe discharge treatment for biological laboratory wastewater and holds significant application potential.