微生物固定CO₂ 是一种极富前景的碳捕获和利用途径, 对实现全球生态系统碳中和 至关重要. 非光合自养微生物由于其环境适应性强、固碳途径的多样性, 以及会产生高附加值 产物而备受关注. 基于非光合自养微生物固定CO₂ 的研究进展, 系统分析影响非光合自养微 生物固碳效率的内部和外部因素, 包括有固定CO₂ 途径、固碳关键酶、碳源、电子供体、代谢 产物等, 并列出多种增效策略. 除了直接对应影响因素的增效方法外, 还涉及微生物间的协同 作用及代谢促进剂(生物炭与微纳米气泡) 的使用. 本工作有助于为深入理解和进一步增效非 光合自养菌固定CO₂ 提供理论基础和技术支撑.

Microbial CO₂ fixation is a promising pathway of carbon capture and utilization, which is crucial for achieving carbon neutrality in global ecosystems. Non-photosynthetic autotrophic microorganisms have attracted attention for their environmental adaptability, diversity of carbon fixation pathways, and production of high value-added products. Based on domestic and foreign researchs on CO₂ fixation by non-photosynthetic autotrophic microorganisms, we systematically analyzed internal and external factors affecting the carbon fixation efficiency of non-photosynthetic autotrophic microorganisms, including CO₂ fixation pathways, key carbon fixing enzymes, carbon sources, electron donors, and metabolites. Various strategies for promoting microbial CO₂ fixation have been proposed. In addition to strategies involving direct responses to influencing factors, strategies involving synergy between microorganisms and the use of metabolic promoters (biochar and micro/nanobubbles) are also being investigated. This review provides a theoretical foundation and technical support for enhancing CO₂ fixation by non-photosynthetic autotrophic microorganisms.