收稿日期: 2020-02-11
网络出版日期: 2020-01-07
Degradation characteristics of biodegradation of tetrabromobisphenol A by the novel aerobic strain W1-2
Received date: 2020-02-11
Online published: 2020-01-07
W1-2 菌株是以好氧活性污泥为菌源, 以四溴双酚 A(tetrabromobisphenol A, TBBPA)驯化筛选得到的一株新型好氧降解菌株. 16S rDNA 序列表明, W1-2 菌株属于假单胞菌属(Pseudomonas sp.), 主要以酶降解的模式去除 TBBPA. 在 30 ℃、pH=7、 150 r/min 和无其他碳源辅助的条件下, W1-2 菌株对 10 mg/L TBBPA 5 d 的好氧降解率可达 91.4%. 温度、转速、pH 值及 TBBPA 的质量浓度均会影响 W1-2 菌株的降解特性, 其中 pH 值对降解率的影响最大. W1-2 菌株最适宜降解和生长的环境条件为 150 r/min、30~ 35 ℃, TBBPA 质量浓度为 10 mg/L 和 pH=8. 此外, W1-2 菌株也是为数不多的无需其他碳源支持、能在高 TBBPA 质量浓度(30 mg/L)和低氧(0 r/min)条件下仍保持高降解能力的好氧降解菌株. 对 W1-2 菌株的研究, 为探究好氧环境下能降解 TBBPA 的微生物的修复提供了新的视角.
杨书娴, 胡星 . 新型好氧 W1-2 菌株降解四溴双酚 A 的性能[J]. 上海大学学报(自然科学版), 2022 , 28(1) : 57 -66 . DOI: 10.12066/j.issn.1007-2861.2267
A novel strain, namely W1-2, was acclimatised and isolated from aerobic activated sludge in a mineral salt medium with tetrabromobisphenol A (TBBPA). Strain W1-2 was identified as Pseudomonas sp. according to the 16S rDNA sequence. After 5 days of aerobic incubation, the degradation rate of 10 mg/L TBBPA was 91.4% at 30 ℃, pH=7, and 150 r/min. The temperature, rotation speed, pH and TBBPA concentration could influence the degradation rate, among which pH was the most influential parameter. The optimal conditions for bacterial degradation and the growth of strain W1-2 were 150 r/min, 30~35 ℃, initial TBBPA concentration of 10 mg/L, and pH=8 according to a single-factor experiment. Strain W1-2 had an excellent degradation ability at high levels of TBBPA(30 mg/L) and under micro-oxygen conditions (0 r/min) without the support of other carbon sources. This study on the degradation characteristics of strain W1-2 provides a new perspective to explore the microbial removal of TBBPA in aerobic environments.
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