研究论文

乌江流域某水库浮游藻类群落结构及多样性分析

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  • 1.上海大学 环境与化学工程学院, 上海 200444
    2.天津大学 表层地球系统科学研究院, 天津 300072
杨明(1979---), 女, 研究员, 博士, 研究方向为环境生态学. E-mail: mingyang@shu.edu.cn.

收稿日期: 2018-07-09

  网络出版日期: 2018-09-14

基金资助

国家重点研发计划资助项目(2016YFA0601001)

Community structure and diversity phytoplankton in the Wujiang River Basin reservoir

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  • 1. School of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, China
    2. Institute of Surface-Earth System Science, Tianjin University, Tianjin 300072, China

Received date: 2018-07-09

  Online published: 2018-09-14

摘要

浮游藻类在河流生态系统中发挥着重要的生态功能. 为探明乌江流域不同梯级水库中浮 游藻类的种群结构特征, 采集了乌江流域某水库水深分别为 0, 10 和 45 m 的水样过滤膜分层样 本, 利用两对浮游藻类通用简并引物对水样进行 23S rRNA 基因扩增、扩增片段建库和高通量测序分析. 通过对两对引物获得的浮游藻类种群 覆盖度比较, 选择出了相对高效的分子标记, 获得了不同水深水体中浮游藻类群落结构组成的初步信息, 为进一步深入研究乌江流域不同梯级水库中浮游藻 类物种多样性提供了一种新手段.

本文引用格式

岳一鸿, 傅志伟, 陈学萍, 杨明, 王宝利, 汪福顺 . 乌江流域某水库浮游藻类群落结构及多样性分析[J]. 上海大学学报(自然科学版), 2021 , 27(1) : 97 -105 . DOI: 10.12066/j.issn.1007-2861.2085

Abstract

Phytoplankton plays a crucial role in river ecosystems. To characterise the structure of phytoplankton communities in cascade reservoirs of the Wujiang River Basin, filter samples were collected at 0, 10, and 45 m depth, and two previously reported degenerate primers were applied to amplify the 23S rRNA gene fragment and construct the library for high-throughput sequencing. An efficient identification method was developed through comparison of species coverage between two degenerate primers, and primary information was obtained on the phytoplankton community diversity. This study provides a powerful tool for potential studies on the ecological effect of damming on phytoplankton communities in riverine ecosystems.

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