构建封装蛋白质的复合水凝胶微结构在药物缓释、生物支架改性修饰等领域具有重要应用. 报告了一种基于光诱导电聚合原理的免疫球蛋白/聚乙二醇二丙烯酸酯(immunoglobulin G/polyethylene glycol diacrylate, IgG/PEGDA)复合水凝胶微结构的图形化加工方法. 首先, 对 PEGDA 光诱导电聚合的图形化加工原理进行了研究, 并分别对单纯 PEGDA 水凝胶和包裹 IgG 蛋白质的复合 PEGDA 水凝胶的微结构光诱导电聚合图形化加工进行了实验验证. 实验结果表明, 所提出方法可以利用水凝胶分子和蛋白质的混合溶液, 结合电脑光斑图形和交流电场加工多种几何形状的蛋白质复合水凝胶微结构. 在加工过程中, 水凝胶微结构的截面形状由光斑图形控制, 水凝胶微结构的高度随着沉积时间的增加而增加, 最小加工尺寸为 3~4 μm. 利用所提出方法可以实现传统微加工方法难以实现的管状、高深宽比的复合水凝胶微结构加工.

Encapsulating proteins in hydrogels has wide applications in drug release and tissue scaffolds modifications. In this paper, an optically-induced electropolymerization technique for patterning immunoglobulin G/polyethylene glycol diacrylate (IgG/PEGDA) hydrogels is reported. First the mechanism of optically-induced electropolymerization of PEGDA hydrogels is investigated. Then the electropolymerization of pure PEGDA and IgG/PEGDA hydrogels are experimentally verified respectively. The experimental results indicate that the reported technique can manufacture protein-encapsulated hydrogels with different geometry shapes, through using optical images designed by computer, alternating electrical field, and solution with hydrogel molecules and proteins. The cross-section shapes of the hydrogels are controlled by the optical images, the height of the hydrogels becomes increased with the increase of the deposition time. The smallest size of the manufactured structures is 3~4 μm. This technique makes possible the manufacture of tubular and high depth/width-ratio hydrogel microstructures, which is hard to imagine with the traditional manufacture methods.