Non-enzymatic glucose biosensor based on Ag/AgCl/Polydopamine-Graphene composite
Received date: 2017-04-12
Online published: 2019-12-31
利用多巴胺的自聚性能及还原性, 采用一步法制备聚多巴胺/银复合物修饰的还原石墨烯复合纳米材料. 采用的工作电极是玻碳电极, 将制备的复合纳米材料滴涂在其表面, 构建了无酶葡萄糖电化学传感器 (Ag/AgCl/PDA-rGO). 材料的结构和组成借助X射线衍射、拉曼光谱等仪器进行了表征和分析, 表明本方法成功地将聚多巴胺和银复合物均匀地负载于还原石墨烯的表面, 同时通过调节反应时间, 改变和控制 Ag/AgCl/PDA-rGO 材料中聚多巴胺和银复合物的负载量. 此外, 使用交流阻抗技术和循环伏安法分析和研究了 Ag/AgCl/PDA-rGO 修饰电极的电化学性能. 试验结果表明, Ag/AgCl/PDA-rGO 构建的无酶葡萄糖传感器在葡萄糖浓度为 5$\sim $9 mmol/L 时, 线性关系良好.
王瑞, 胡亦杨, 蒋晓濛, 赵攀登, 程伶俐, 焦正 . Ag/AgCl/PDA-rGO 无酶葡萄糖传感器的构建[J]. 上海大学学报(自然科学版), 2019 , 25(6) : 943 -949 . DOI: 10.12066/j.issn.1007-2861.1947
According to self-polymerization property of dopamine and reductibility, polydopamine/silver composite/graphene was synthesized in one step. In addition, the graphene compound fixing on the surface of glassy carbon electron as a working electrode was used to construct a non-enzymatic glucose sensor. With a series of characterization methods such as X-ray power diffraction (XRD) and Raman spectrum, polydopamine/silver composite/graphene is synthesized successfully. Further, concentration of polydopamine/silver composite can be controlled by the reactive time. Electrochemical performance was tested with electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). The biosensor has shown a good linear relationship under the condition that concentration of glucose is between 5 mmol/L and 9 mmol/L
Key words： graphene oxide; dopamine; Ag; glucose sensor
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