CdTe量子点荧光量子产率及生物标记

上海大学学报(自然科学版) ›› 2009, Vol. 15 ›› Issue (2): 142-146.

CdTe量子点荧光量子产率及生物标记

陈丽霞1,2，张建成2，宋振伟2，尤陈霞2，丁益民1，沈悦2，郭景康3

（1.上海大学理学院,上海 200444; 2.上海大学材料科学与工程学院,上海 200072; 3.上海大学生命科学学院,上海 200444)

收稿日期:2007-12-02 出版日期:2009-04-22 发布日期:2009-04-21
作者简介:张建成(1948～)，男，教授，研究方向为低维功能材料. Email:jchzhang@mail.shu.edu.cn
基金资助:
国家自然科学基金资助项目(90206017)；上海市教委基金资助项目（08YZ08）；上海市科委基金资助项目（08520741600，0752nm016,07JC14058）;上海大学系统生物研究基金资助项目

CdTe Quantum Dots Fluorescence-Quantum Yield and Biological Labeling

CHEN Li-xia1,2,ZHANG Jian-cheng2,SONG Zhen-wei2,YOU Chen-xia2,
DING Yi-min3,SHEN Yue2,GUO Jing-kang3

(1. College of Sciences, Shanghai University, Shanghai 200444, China; 
2. School of Materials Science and Engineering, Shanghai University, Shanghai 200072, China; 
3. School of Life Sciences, Shanghai University, Shanghai 200444, China)

Received:2007-12-02 Online:2009-04-22 Published:2009-04-21

摘要/Abstract

摘要：

采用巯基乙酸为稳定剂,在水相中直接合成CdTe量子点，实现半导体纳米晶体的水溶性.利用巯基乙酸包覆的CdTe量子点外端的羧基与牛血清白蛋白的氨基，通过偶联剂的作用形成酰胺键，从而达到标记蛋白质的作用.利用荧光和紫外可见分光光度计,研究标记前后体系的发射光谱和吸收光谱的变化以及荧光量子产率，通过透析膜对标记后的牛血清白蛋白进行纯化，进一步用琼脂糖电泳证明牛血清白蛋白与CdTe量子点的结合.

关键词: 量子点；荧光量子产率；牛血清白蛋白；生物荧光标记

Abstract:

CdTe quantum dots (QDs) were synthesized in an aqueous solution with thioglycolic acid (TGA) as a stabilizing agent to actualize water solubility of semiconductor nanocrystals. The carboxylic acid groups of thioglycolic acid (TGA) stabilizing CdTe quantum dots can form amido bonds with the amine groups of bovine serum albumin by the coupling action to label the protein.Before and after labeling bovine serum albumin (BSA), the change of emission and absorption spectra of CdTe quantum dots as well as their fluorescence-quantum yield were studied by fluorescence and UV-vis spectrophotometer. CdTe-BSA was confirmed by gel electrophoresis after being purified by dialysis.

Key words: quantum dots; fluorescence-quantum yield; bovine serum albumin (BSA); biological fluorescent labeling

中图分类号:

O 614.2

陈丽霞张建成宋振伟尤陈霞丁益民沈悦郭景康. CdTe量子点荧光量子产率及生物标记[J]. 上海大学学报(自然科学版), 2009, 15(2): 142-146.

CHEN Li-Xia, ZHANG Jian-Cheng, SONG Zhen-Wei, YOU Chen-Xia, DING Yi-Min, SHEN Yue, GUO Jing-Kang. CdTe Quantum Dots Fluorescence-Quantum Yield and Biological Labeling[J]. Journal of Shanghai University（Natural Science Edition）, 2009, 15(2): 142-146.