中药提取物对酵母和鼠肠α-葡萄糖苷酶的抑制作用

上海大学学报(自然科学版) ›› 2009, Vol. 15 ›› Issue (4): 432-435.

中药提取物对酵母和鼠肠α-葡萄糖苷酶的抑制作用

司晓晶霍世欣施雅吕敬慈

上海大学理学院，上海 200444

出版日期:2009-08-30 发布日期:2009-08-30
通讯作者: 吕敬慈(1947～)，男，教授，研究方向为天然活性物质的分离分析和糖尿病新药. E-mail:jclu@mail.shu.edu.cn

Inhibition of Traditional Chinese Medicine Extracts on α-Glucosidase from Yeast and Rat Intestines

College of Sciences, Shanghai University, Shanghai 200444, China

Online:2009-08-30 Published:2009-08-30

摘要/Abstract

摘要：

从治疗糖尿病的药方中选出常用52种中药，采用水煎煮，水煎煮后的残渣用60%～70％的乙醇提取以及直接用60％的乙醇提取的方法得到三部分提取物，并用α-葡萄糖苷酶活性实验进行筛选,研究各种中药提取物对鼠肠和酵母α-葡萄糖苷酶抑制作用.发现桑枝、覆盆子等中药分别对鼠肠和酵母α-葡萄糖苷酶有明显的抑制作用；桑白皮等4种中药的水煎煮后乙醇提取物对鼠肠和酵母α-葡萄糖苷酶均有很高的抑制作用，其对酵母α-葡萄糖苷酶抑制类型为非竞争抑制，Km=4.352×10-4 mmol·L-4.实验结果对开发研究治疗糖尿病的新型药物具有很好的参考价值.

关键词: α-葡萄糖苷酶；抑制剂；筛选；中草药；糖尿病

Abstract:

Based on resources of the traditional Chinese medicine (TCM) and large amount of prescriptions for diabetic treatment, 52 medicines were selected, and their aqueous extracts, ethanol extracts (60%~70%) of residues after aqueous extracts and ethanol extracts were tested respectively using α-glucosidase activity assays. Their α-glucosidase inhibition effect from rat small intestines and yeast are compared. TCM including mulberry stalk and raspberry have remarkable inhibition effect on rat’s small intestines and yeast at the concentration of 1 mg·mL-1. Four TCM (thanol extracts of residues after aqueous extracts) including Cortex Mori have strong inhibition both on yeast and ratintestines α-glucosidases. Kinetics of enzyme-catalyzed reactions of Cortex Mori was taken. The inhibition type is noncompetitive inhibition, Km=4.352×10-4 mmol·L-1. The results are valuable in research and discovery of new medicines for treatment of diabetes mellitus.

Key words: α-glucosidase; inhibitior; screening; traditional Chinese medicine (TCM); diabetes mellitus

中图分类号:

Q556+.2

司晓晶霍世欣施雅吕敬慈. 中药提取物对酵母和鼠肠α-葡萄糖苷酶的抑制作用[J]. 上海大学学报(自然科学版), 2009, 15(4): 432-435.

SI Xiao-Jing, HUO Shi-Xin, SHI Ya, LU Jing-Ci. Inhibition of Traditional Chinese Medicine Extracts on α-Glucosidase from Yeast and Rat Intestines[J]. Journal of Shanghai University（Natural Science Edition）, 2009, 15(4): 432-435.