拟南芥温敏雄性不育突变体atms1的获得及表型分析

doi:10.3969/j.issn.1007-2861.2011.05.020

上海大学学报(自然科学版) ›› 2011, Vol. 17 ›› Issue (5): 681-686.doi: 10.3969/j.issn.1007-2861.2011.05.020

拟南芥温敏雄性不育突变体atms1的获得及表型分析

戴文懿，孙亚梅，高贝，周树敏，袁晓君，韦嘉励，张卫

上海大学生命科学学院，上海 200444

收稿日期:2010-05-04 出版日期:2011-10-26 发布日期:2011-10-26
通讯作者: 张〓卫（1966~），男，教授，博士，研究方向为拟南芥雄性不育等. E-mail:wzhang1216@yahoo.com E-mail:wzhang1216@yahoo.com
基金资助:
上海市科委浦江人才计划资助项目（08PJ1405500）；上海市优秀青年教师科研专项基金资助项目

Thermo-Sensitive Male Sterile Mutant atms1 of Arabidopsis thaliana Access and Phenotypic Analysis

DAI Wen-yi,SUN Ya-mei,GAO Bei,ZHOU Shu-min,YUAN Xiao-jun,WEI Jia-li,ZHANG Wei

School of Life Sciences, Shanghai University, Shanghai 200444, China

Received:2010-05-04 Online:2011-10-26 Published:2011-10-26

摘要/Abstract

摘要： 从甲基磺酸乙酯（ethylmethane sulfonate,EMS）化学诱变建立的野生型拟南芥（Col-0）突变体文库中筛选到1株突变体.在低温16 ℃时，该突变体的雄性育性与野生型没有显著差异，花粉染色呈现100%可育.随着培养环境温度的升高，突变体花粉育性逐渐下降，因此，该突变体为一温敏雄性不育突变体,并被命名为atms1（ambient temperature-sensory male sterility 1，即环境温度敏感雄性不育1）.花药切片结果显示，在23 ℃以下，该突变体花药各个发育时期的形态与野生型花药没有显著的差异；而27 ℃处理1周后的突变体花药呈现多种表型：同一朵花中各个花药的发育时期出现显著分化，花粉母细胞胼胝体单薄，绒毡层发育滞后于同时期的野生型花药.遗传分析确定，atms1的不育表型是由单个隐性核基因控制的.

关键词: 花粉, 花药, 拟南芥, 绒毡层, 温敏雄性不育

Abstract: An ambient temperature Arabidopsis thaliana male sterile mutant ambient temperature-sensory male sterile 1 (atms1), generated from ethylmethane sulfonate (EMS) mutagenesis has been identified. At low temperature 16 ℃, atms1 showed 100% pollen viability and silique fertility, while with the culture temperature increased, atms1 showed decreasing pollen viability and silique fertility. Therefore atms1 is a temperature sensory male sterile mutant. The morphological assay demonstrated that atms1 anther showed few significant impairments compared with wild-type anther when ambient temperature was below 23 ℃. At higher temperature 27 ℃, the atms1 anther exhibited pleiotropic phenotypes: the developmental phases of anthers within one flower differed significantly; the callose layer of pollen mother cell of mutant was much thinner than that of wild-type, and the atms1 tapetum development exhibited much retarded than corresponding wild-type tapetum. Genetic assay demonstrated that the phenotypes of atms1 were controlled by a single recessive locus.

Key words: Arabidopsis thaliana, anther, pollen, tapetum, temperature-sensory male sterility

中图分类号:

Q 94

戴文懿，孙亚梅，高贝，周树敏，袁晓君，韦嘉励，张卫. 拟南芥温敏雄性不育突变体atms1的获得及表型分析[J]. 上海大学学报(自然科学版), 2011, 17(5): 681-686.

DAI Wen-yi,SUN Ya-mei,GAO Bei,ZHOU Shu-min,YUAN Xiao-jun,WEI Jia-li,ZHANG Wei. Thermo-Sensitive Male Sterile Mutant atms1 of Arabidopsis thaliana Access and Phenotypic Analysis[J]. Journal of Shanghai University（Natural Science Edition）, 2011, 17(5): 681-686.

拟南芥温敏雄性不育突变体atms1的获得及表型分析

Thermo-Sensitive Male Sterile Mutant atms1 of Arabidopsis thaliana Access and Phenotypic Analysis

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