发酵乳杆菌 B44 益生性能的体外评价

doi:10.12066/j.issn.1007-2861.2207

上海大学学报(自然科学版) ›› 2021, Vol. 27 ›› Issue (6): 1162-1170.doi: 10.12066/j.issn.1007-2861.2207

• 研究论文 • 上一篇

发酵乳杆菌 B44 益生性能的体外评价

韩瑨¹, 吴燕婷¹^,², 万嗣宝², 吴正钧¹()

1.光明乳业股份有限公司乳业研究院, 上海 200436
2.上海大学生命科学学院, 上海 200444

收稿日期:2019-05-21 出版日期:2021-12-31 发布日期:2022-01-06
通讯作者: 吴正钧 E-mail:wuzhengjun@brightdairy.com
作者简介:吴正钧(1966—), 男, 教授, 博士生导师, 研究方向为乳品发酵技术. E-mail: wuzhengjun@brightdairy.com
基金资助:
上海乳业生物工程技术研究中心资助项目(19DZ2281400)

In vitro evaluation of the prebiotic properties for Lactobacillus fermentum B44

HAN Jin¹, WU Yanting¹^,², WAN Sibao², WU Zhengjun¹()

1. Dairy Research Institute, Bright Dairy Co., Ltd. Shanghai 200436
2. College of Life Sciences, Shanghai University, Shanghai 200444, China

Received:2019-05-21 Online:2021-12-31 Published:2022-01-06
Contact: WU Zhengjun E-mail:wuzhengjun@brightdairy.com

摘要/Abstract

摘要：

发酵乳杆菌 B44 是一株分离自保加利亚家庭手工制作酸奶的乳酸菌株. 体外实验发现: 发酵乳杆菌 B44 具有较强的疏水性(79.67%)和低酸高碱的静电作用, 可耐受 1 mg/mL 溶菌酶, 在口腔中具有较佳的黏附和存活能力; 此外, 具有较高的自聚率(13.34%)以及与变形链球菌(Streptococcus mutans)的共聚集作用, 当与变形链球菌共培养时能抑制变形链球菌生物膜的形成, 其抑制率可达 63.43%. 实验表明, 发酵乳杆菌 B44 对人类龋病的防治能起到一定的积极作用, 是一株潜在的口腔益生菌, 在人类口腔健康中具有良好的应用前景.

关键词: 益生菌, 变形链球菌, 生物膜, 龋病, 共聚

Abstract:

Lactobacillus fermentum B44 is a lactic acid strain isolated from Bulgarian homemade yoghurt. In vitro experiments showed that L. fermentum B44 has strong hydrophobicity (79.67%) and an electrostatic effect of low acid and high alkali; the strain could tolerate 1 mg/mL lysozyme and has a better adhesion and survival ability in oral cavities. In addition, it also has a high self-agglomeration rate (13.34%) and strong co-aggregation with Streptococcus mutans. When co-cultured with S. mutans, the formation of S. mutans biofilm was inhibited, and the inhibition rate was 63.43%. The above results showed that L. fermentum B44 could play a positive role in preventing and treating human dental caries; it is a potential oral probiotic and has a suitable application prospect in human oral health.

Key words: probiotics, Streptococcus mutans, biofilm, caries disease, co-aggregation

中图分类号:

韩瑨, 吴燕婷, 万嗣宝, 吴正钧. 发酵乳杆菌 B44 益生性能的体外评价[J]. 上海大学学报(自然科学版), 2021, 27(6): 1162-1170.

HAN Jin, WU Yanting, WAN Sibao, WU Zhengjun. In vitro evaluation of the prebiotic properties for Lactobacillus fermentum B44[J]. Journal of Shanghai University（Natural Science Edition）, 2021, 27(6): 1162-1170.

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发酵乳杆菌 B44 益生性能的体外评价

In vitro evaluation of the prebiotic properties for Lactobacillus fermentum B44

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