Abstract:

[Objective] To develop an optimized method which is more stable and efficient in hepatic telocytes separation based on current method, and to establish a solid theoretical foundation and experimental support for further study of potential regulation of hepatic cells by telocytes. [Methods] C57BL/6 male wild mice were selected for isolation of liver telocytes through multiple short enzymatic digestions and centrifugation. CD34+Vimentin immunofluorescence staining was used to identify the cell phenotype. [Results] Hepatic telocytes were successfully separated. Hepatic telocytes were both CD34 and Vimentin positive in vitro, while hepatic stellate cell line was CD34 negative and Vimentin positive. [Conclusion] High-quality telocytes were obtained through this optimized method and could be used for other studies.

Key words: telocytes(TCs), cell isolation, ultrastructures, immunophenotypes